Connected on 2014-05-12 13:00:00
from Orange County, California, United States
- 11:46 am
- Bugscope Teamsample is in 'scope and pumping down
- 11:56 am
- 12:03 pm
- 12:09 pm
- 12:15 pm
- 12:20 pm
- 12:26 pm
- 12:33 pm
- 12:40 pm
- 12:47 pm
- TeacherHello Scot!
- Bugscope Teamhello!
- TeacherSo excited! I have 19 students waiting already :)
- Bugscope Teamwe are just finishing up now
- Teacherok
- Bugscope Teamwe can start early if you want. we are ready to go
- TeacherI prefer to wait until 11am so I don't miss any students who want to see :) thanks
- TeacherDid you get a lot of samples from us?
Bugscope TeamI don't believe we got anything,
- Teacherawwwww!! Some students told me they sent some in...
- Bugscope TeamI checked a little while ago.
- Teacherso glad you have extras - thanks a bunch!
- Bugscope Teamoh yeah this is a good sample -- Cate made it
- Teachergreat!
- Bugscope TeamMrs Evans (this is Scot, in my office) you have control of the 'scope if you would like to try driving.
- 12:53 pm
- Bugscope Teamyou can see that we made presets, available for clicking on the lefthand screen
- Bugscope Teamyou can also change the mag, drive by using click to center on the screen, change focus, etc.
- Bugscope TeamI just moved us to one of the presets
- Bugscope Teamthis is a baby ladybug, so cute
- Teacherclicking on the focus + sign to make it more clear
- Bugscope Teamto the lower left we see a small mess of limbs; ladybug larvae eat aphids and leave them on their bodies, sometimes
- Teachermy students can adjust the controls too, right?
Bugscope Teamif they are able to log in, we can confer control to them if you wanted
- Teachervery cool!
- Teacherok thank you, I will let you know who
- Bugscope Teamthe way focus works with this microscope is you go up or down. so if you click in the plus direction and it gets worse, click in the minus direction
- Teacherok
- 12:59 pm
- Teacherok
- TeacherCool!
- Teacherhi everyone
- Teacherhi
- TeacherHi everyone!
Bugscope Teamelcome to Bugscope!
Bugscope Teamhaha
- Teacherhey guys
- Teacheri didn't see for student or not
- GuestHello :)
- Teacheromg what is that
Bugscope Teamwe are looking at a baby ladybug. isnt it cute?!
- Teacherhiiiii
- Bugscope Teamthis is the head of a ladybug larva
- Teacherthis is awesome!
- Teacheri just hit enter
- TeacherNow you know my full name :(
- Teacherhii
- GuestAre w Here?
Bugscope Teamwe are here!
- Teacherthat is scary
- Teacherwhat is that?
- Teacherwats that creature!
Bugscope Teamit's a ladybug larva
- Guesti like thiis
- Teacherno
- GuestThat is disturbing
- Teacheroh
- Teacherhi
- Teacherlol
- GuestMY WASP CAME yay
- Teacheri wont be sleeping tonight
- Bugscope Teamwe can see its palps -- the conical things. and we can see one of its mandibles
- Teacherthe screen is staying the same
- Teachernow it changed
- TeacherI never knew mosquitoes had scales
Bugscope Teamthe scales protect them from getting caught in spiderwebs
- Teacheryou're changing the pictures too fast
- GuestIs that like a tongue..
- Teacherthis is awesome!
- StudentHello
- Teacherlogged in
- Bugscope Teamthe forked thing we see here is one of the mandibles -- one of the jaws
- Teacheryuc
- Teacherdid i disconnect?
- Teachergross
- Teacherdo thay have rocahes
- Teacheri am looking at the ladybug larva
- Bugscope Teamthe mandibles open left and right, like a gate
- TeacherIm logged innn
- Teacherthe lady bug larvae looks like a sock pocket
- Teacherthis is amazing
- Teachermorgan
- Teacher100 microns
- 1:04 pm
- Teacheroooooooooooooooooooooooooooooo
- Teacherwhat does it mean by Microns?
Bugscope Teamthose are micrometers. they are a millionth of a meter
- Teacherim hungryyy
- TeacherMelody would like control of the microscope
Bugscope Teammelody has control
- Teacherwhat does it mean by microns?
- TeacherSuper cool
- Teacherwe had to send a bug in? i didn't know that
- GuestHow do i look at the Spider?
Bugscope Teamsomeone who has control of the 'scope has to click on that preset
- Teacherooo
- Teacherthat will come with the future
- Teacherwait...send in a bug? what does that mean?
- Teachercan we see Wendy's salt?
- Teacherwhy is it black and white?
Bugscope Teamwe are not using light to collect these images, which are live images from the scanning electron microscope
- Teacherwhat does it mean by microns?
Bugscope Teama micron is the same as a micrometer, which is one thousandth of a millimeter, a millionth of a meter, and 1000 nanometers
- TeacherAshley, my Bio students sent in bugs for today's session.
- StudentGino, GonzalezWhy is there no color?ยป So do the incects not preciev color atr all?
Bugscope Teamthey had color. we coated them with a metal alloy to make them conductive, so after that they were grey. The electrons are smaller than light waves as well, which is what we are using to image. This microscope can see things as small as 2 nanometers! Visible light is around 400-700 nanometers
- Teachersweet!
- Guestpikachu
- Teacheryikes
- Teacheroooh
- Teachernevermind
- Bugscope Teamaphid on a cell phone
- TeacherAphid - cool!
- Teachercreepy
- TeacherMy screen isn't changing..
Bugscope Teamplease hit refresh on your browser, see if that works
- Teacherhow long is this session?
- Teachergosh these images are making me itching and i'm not sleeping tonight or ever
- 1:09 pm
- Teacherthat long thing coming down from its face...is that its mouth or nose
- TeacherWhat happens in you scan a alive insect?
Bugscope Teamthe live insect would have to hold very still. also, it would be inside the vacuum chamber and not very pleased about that, plus it would normally be coated with gold-palladium, as these insects are
- Teacherhow long is This session?
Bugscope Teamum until 12 your time
- Teacheri see bugs with abs
Bugscope Teamhaha Yeah!
- Teacheris that why they are cracked
Bugscope Teamthey crack when they dry, or sometimes when we handle them -- they are actually very small\
- Teacherdoes SJ work here?
Bugscope TeamI work here in the Underworld, in Illinois
- Bugscope Teamsj is scott robinson. we works here in a lab at the university of illinois
- Teacheri am
- Teacherhow was CalPac able to set this up??
Bugscope Teamyou fill out an application online so we can set up a time
- TeacherSince they went to a drying out process one of the aphid has cracks in it's body. Right?
Bugscope Teamthe aphid was indeed subjected to critical point drying; otherwise it would have been totally shriveled up
- Teacherwhat is the question
- TeacherIt's so cool that you do this
- Teachercrazy stuff :D
- TeacherDo they have wings? It looks like they have wings.
Bugscope Teamthey have wings, and that is kind of cool because it tells us that the preceding generation was stressed for food, so this generation was born with wings
- Teacher1 hour
- Teacheri accidentally went in as teacher
- Teachercrazy stuff :D
- Teachercrazy stuff :D
- TeacherWhat is the conversion from Microns to centimeters.
Bugscope Teamthere are 10,000 micrometers in a centimeter
- Guestcan insects be dried up but alive
Bugscope Teamno. it would die. just like humans can die from dehydration
- 1:15 pm
- Teacheryou dry out the insect?
- TeacherTest
- Teacherthis is cool
- GuestHow long does it Tend to Drain a 6 CM Blackwidow
- TeacherWhat do you use for drying out the insects???
Bugscope Teamwe usually air dry, but sometimes we use a special machine called a critical point dryer. The critical point dryer will use liquid carbon dioxide and heating to specially dry the insects so that they can retain their shape.
- Teachercan Morgan have control?
Bugscope Teammorgan has control
- Bugscope Teamoops I am sorry I just wiped Morgan out by accident -- my fault
- Teacherwhen they are dried, are their organs kept in tact or did they shrivel up?
Bugscope Teamthey shrivel up. When bug experts want to see their organs, they have to do it when the insect is still fresh I think
- TeacherI dont get this thing
Bugscope Teamwhat don't you get?
- Bugscope Teamask Morgan if she can log in again; I'm sorry
- TeacherWhat do you call the tiny hair on the insects?
Bugscope Teamthey are called 'setae,' pronounced see-tee
- Teacherim in still!
Bugscope Teamyay!
- Bugscope Teamthe setae are also called trichae, microtrichae, spines, bristles...
- Teacheri got kicked out sorry
- 1:21 pm
- Bugscope Teamwe are running into intermittent lag with the connection
- Teacherwhat is that?^
Bugscope Teamthis we are not sure but believe it is a wasp
- Teachertest
- TeacherWhy do all the bug's eyes look the same?
Bugscope Teamthey may appear similar and act in similar functions, but there are slight variations. Like flying insects usually have large round compound eyes, while insects that living in the dirt, like ants, have smaller, and sometimes even nonexistent, compound eyes
- Bugscope Teamsome flying insects also have three simple eyes, on the top of the head, called ocelli
- Teachertest
- Teacherdo insects see in color?
Bugscope Teamyes but not always the same colors we do; they can also, often, see in ultraviolet wavelengths, which would require us to use a blacklight to see
- TeacherWhy are the legs so hairy?
Bugscope Teamthe hairs, which we call setae since the hairs aren't mammalian, are to help the insect to sense what is going on around it. Most of the hairs you see are for sense of touch
- Teacheris it like cat wiskers
Bugscope Teamexactly!
Bugscope Teamlike cat whiskers that can also smell and sense hot/cold
- Teacherhow are we able to tell if they see in color or not? is there a special lens that scientists observe?
Bugscope TeamI think they have done experiments with color, and they have also mocked up how they expect the eyes to work given the way they are set up
- 1:26 pm
- Guestthis was cool
- Bugscope Teamit is rare that we are actually able to see into the cavity of the mouth with any insect
- Bugscope Teamhere we see a set of mandibles as well
- Studentfinally back online
Bugscope TeamYay!
- Teacherhey i am back
Bugscope TeamSuper cool.
- Teacherhi
Bugscope TeamHello!
- Guestit's still frozen for me
- GuestCan Abigail please have control of the microscope?
Bugscope Teamshe has control
- Teacheramazing
- TeacherSome of the bugs have lumps on then that look like dirt but what really is it? I'm guessing bacteria.
Bugscope Teamusually it's dust or dirt
- Bugscope Teamwe can see bacteria in a cluster above
- Bugscope Teamdefinitely bacteria this time
- Teacherdo they have hair on their wings?
Bugscope Teamoften they do; the hairs, in this case microsetae, likely help them hold onto the air
- Teacherhi
- Bugscope Teamit appears, also, that having tiny setae on the wings likely helps if they get stuck to a wet surface
- 1:32 pm
- Teacheramazing
- TeacherDo lady bugs have teeth?
Bugscope Teamno. they have mandibles, though. they act like teeth to chew up plants.
- GuestWe are having some issues with students and teachers being able to log in. Is there a way to fix that problem?
Bugscope Teamit could be that, because there are so many people logged in, it is taking a bit longer for some other people to log in now. everything is lagging up a little
Bugscope Team do with available bandwidth; we have had problems with it before
- Guestcould we please see another bug?
- TeacherCool, thank you!
- GuestI'm having issues with the chat
- Teacherhi ms.evans :D
- Studenthow is the microscope able to focus on everything?
Bugscope Teamit uses electromagnets to control and focus the beam
Bugscope Teamthe reason the focus looks good, often, is because the electron beam is very fine and thus things that might a little out of focus look good until we get still closer
- Teacherwhat kind of bug is that?
Bugscope Teamit is a type of borer
- 1:37 pm
- GuestCan we see the slide of the crystals please?
Bugscope Teamtechnically, to clarify, this is not a slide but a live image from the scanning electron microscope
- TeacherIs that the bacteria on a crystal or the actual crystal?
- Teacherhow long will it take to dry out a 6 CM Blackwidow?
Bugscope Teamit would take a while to airdry, but to critical point dry, as long as we can fit it in the chamber, should take around an hour
- GuestWhat kind of crystals were these?
- Bugscope Teamwe think that these crystals formed fairly rapidly, and that is why they are not sharp and well-formed
- TeacherWhat kind of crystals are these?
Bugscope Teamwe do not know about these
- GuestWhat color were the crystals?
Bugscope Teamnot sure. they are pretty small, so we didn't notice them before we coated the insects with metal
- GuestWhy do the crystals have holes?
Bugscope Teamthey are nanocrystals, and they formed apparently very rapidly, so they are not pretty and have gaps in them
- Teacheris there any actual microscopes that can scan live things without doing all the things you have to do to an insect?
- Bugscope Teamhere is another set of crystals
- TeacherSalt in our eyes look circular but in a microscope id looks like cubes.
- Bugscope Teamthese are also odd in that they have a substructure that we think comes from an anticaking agent or maybe a different level of iodination
- Bugscope Teamthese are sodium chloride
- 1:42 pm
- Bugscope Teamwe can see where the antenna broke off, and we can see the compound eye
- GuestDoes it have a compound eye?
- Teacheris there any actual microscopes that can scan live things without doing all the things you have to do to an insect?
Bugscope Teamnot with this kind of resolution
- GuestDoes it have scales?
Bugscope Teamnot really, not like insect scales like on butterflies
- TeacherThe eyes aren't the same as the compoud eyes so what kind of eyes are they?
Bugscope Teamit is a different form of compound eye
- TeacherIt doesn't have setae so does it not feel anything?
Bugscope Teamthey have some, but definitely not as much as some other bugs. Likely they have become really streamlined over time because they have been alive for so long, like roache
Bugscope Teamroaches
- Teacherwhat are those little bubble things by the eye?
Bugscope Teamthose are the individual ommatidia, facets, of the compound eye
- Teacherwhat do they eat?
Bugscope TeamI think these eat decaying organic matter
- GuestWhat type of teeth does it have?
Bugscope Teaminsects and similar arthropods do not have teeth, but they may have hardened mandibles
Bugscope Teamwe find that the mandibles sometimes have zinc or calcium within them
- 1:47 pm
- GuestMay Vanessa please have access to the microscope?
Bugscope Teamvanessa has control!
- TeacherHave you ever had an Insect that was abnormal like a mixture of two other ones?
Bugscope Teamno i don't think so...
- Teacher:D
- Teacheryes
- Bugscope Teamthis is a honeybee head
- Bugscope Teamyou can see one of the mandibles
- TeacherCan a Spider still Eject Venom evene when it's dead? Or does it dry out with the Drying Process?
Bugscope Teamit dries out pretty quickly
- Bugscope Teamthe entomologists have told us that only bees, of all insects, have forked setae
- Teacherwoah O.o
- Bugscope Teamlike this
- Bugscope TeamI'm sitting at the microscope...
- 1:53 pm
- Teacherare the microscopes noisy?
Bugscope Teamthe mechanical pumps used on these microscopes can be, but we keep them in a cabinet
- GuestWhy are they the only one with the forked setae?
Bugscope Teamthey must think they evolved that way, perhaps to better collect pollen.
- TeacherWhere's its nose?
Bugscope Teaminsects do not have noses, unless you count the spiracles, which are on opposing sides of each body segment and through which they breathe; they smell, usually, via the chemosensory setae
- Bugscope Teamthe little pore we see now is called an anterior tentorial pit; it doesn't go anywhere
- GuestWhy do bees sting?
Bugscope Teamit's a protective measure. (unless we are talking about the more agressive ones)
- Bugscope Teamwasps may sting for a different purpose -- to lay their eggs in the host
- GuestHow big is the microscopes??
Bugscope Teamit's about the size of a large refrigerator box
- Teacherdo*
- 1:58 pm
- Teachero you know why or how Spiders know How Much Venom to put into a thing?
Bugscope Teamgreat question. probably, first, they want to immobilize their prey, and they can tell as it quits moving if there is enough
- TeacherThe ant has only one atena. why?
Bugscope Teamthe other one broke off. most likely after it died when it dried out. their extremities are fragile and can break easily
- Teacherof*
- Teacherwhat do ants eat?
Bugscope Teamthey like sweet stuff, kind of like we do, but they will eat anything edible
- Teacherare fly eyeballs made off blood?
Bugscope Teamthey are made of chitin and also crystalline material, not sure what kind
- Teacherthought*
- GuestThank you so much for your time with us today. We enjoyed all of the bugs!
- GuestIs the ant eating something?
Bugscope Teamit looks like it, doesn't it? those are accessory mouthparts called palps that help it taste and also manipulate its food
Bugscope Teamit is hard to tell from here, but some of the critters we are looking at are very small and hard to handle, even with forceps
- Teacheroh i though it as blood since all red
Bugscope Teaminsect blood is called hemolymph, and it is usually clear; when you squish an insect and see other colors they likely come from its gut
- Bugscope TeamThank You, Everyone!
- Teacherthank you
Bugscope TeamThank you, RR
- 2:04 pm
- Teacherthank you! this was very informations and educational
Bugscope Teamwe enjoyed working with you, and we appreciate all of your questions; this is super fun for us
- Teacher:0
- Teacher:)
- Bugscope Teamthis is the inside of the sample chamber
- Teacherwoah!
- Bugscope Teamit is under a vacuum. we can see the insects on the platter to the bottom of the image
- Bugscope Teamthe electron beam comes from the cone, above
- Teacherthat is amazing!
- Bugscope Teamyou can see a fuzzy cage, just barely, in the top right corner. that is the secondary electron detector that the images come from
- Bugscope Teamhttps://bugscope.beckman.illinois.edu/members/2014-006
- Teacherwoah
- Bugscope Teamtime for us to shut down... see you next year!
- Teacherbye
- Bugscope Teamand thank you again. B Ye!
