South Mecklenburg High School
Session 2008-134

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Class

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Transcript

Connected on 2009-03-04 09:45:00 from , NC, US

• 9:45 am
• Bugscope TeamKaren!

• Bugscope TeamWelcome to Bugscope!
• Bugscope TeamWhere are you from?

• Bugscope TeamAre you working with Chad Kelley?

• 9:50 am

• GuestI am actually his wife just logging on from work to see his session!
• Bugscope Teamhe just emailed saying they are experiencing internet problems, but is working on it

• Bugscope TeamAnnie!

• Bugscope Teamhi hi

• 9:55 am

• GuestI see. Hopefully they will get it worked out. He's very excited!

• Bugscope TeamKaren in the meantime we are driving around on the sample; this is the katydid.

• GuestCan it be recorded for him if he can't get his internet problem resolved? The katydid looks great, by the way!

• 10:00 am
• Bugscope TeamAll of the images we get while we are cruising around become part of the database for Chad's session.

• Bugscope TeamChad just wrote and said they are having district wide connectivity issues.
• GuestI just heard from him that the school district is having internet connection issues. I know he's unhappy right now! Oh you just wrote the same thing.
• Bugscope Teamwe are happy to reschedule with him if he wants
• 10:06 am
• Bugscope Teamhello mr. kelley!
• GuestHe will probably like to do that but I would hate to speak for him.
• TeacherI am on!
• Bugscope Teamglad to see you made it!
• Bugscope Teamhey hey!
• Bugscope TeamYay!
• TeacherWe are using the Promethean board as a group. Should we risk dashing back to the media center for group access?
• Bugscope TeamYou can stay logged on here and dash back there -- is it far?
• Teachernot far. Let me see if they have regained internet access. back in a flash.

• 10:13 am
• Bugscope TeamWe can work a little longer today if that is helpful, if that works for you.
• Bugscope TeamMessage from Chad that the system appears to be down again, but he will keep working on it.
• Bugscope Team(via email)

• TeacherTrying again.
• Bugscope Teamhey you are back!
• Bugscope Teamwe just gave you control of the 'scope, if your connection holds
• TeacherWhat have we here?
  Bugscope Teamthis is the millipede you sent us
• Bugscope Teamand we drove you to the millipede
• TeacherThank you!

• 10:18 am
• Bugscope Teamyou can see how its eye is streamlined into its head
• TeacherQuestions about the eye.
• Bugscope Teamthose bumps are the ommatidia

• Bugscope Teamto the left of the base of the antenna
• Bugscope Teamcomparable to the eye of the roly poly
• TeacherNeat. We will try to manify the ommatidia.

• TeacherHow are we doing?
• Bugscope TeamMr K you can choose from among any of the presets along the lower right edge of the screen here, as well
• Bugscope Teamyou are doing great so far
• TeacherOkay.
• Bugscope Teamlooks good -- these eyes are very simple and there may not be much to see beyond this.

• Bugscope Teammoth scales
• TeacherVery nice. A student collected this one!
• Bugscope Teamif you take the mag down you will be able to see where you are on the body

• Bugscope Teammoths and butterflies are covered with scales such as these, and it makes it difficult to coat them adequately for electron microscopy
• Bugscope Teamthis is the proboscis, which is coiled when not being used
• TeacherFunction of the proboscis?
  Bugscope Teamproboscis is like an elephant's trunk
• 10:23 am
• Bugscope Teamit is a hollow tube, and when the moth wants to extend it, it pumps hemolymph into it like a party favor
• Bugscope Teamthe proboscis is what it uses to eat/drink
• Bugscope Teamlike an elephant's trunk, in a way
• Teacherneat.
• TeacherTurning over control to a student. Stay tuned!

• Teacherwhat are those hairs?
  Bugscope Teamthose are exactly what they look like-- hairs
• Bugscope Teamspider head; Cate mounted this one rightside up so we could see all of the eyes
• Bugscope Teambugs are very hairy

• TeacherTell us a little more about the eyes?
• Bugscope Teamspiders often do not use their eyes as much as they may use the hairs -- to sense vibration
• Bugscope Teamthey are used for a variety of things, like to sense vibrations (these here), taste/smell, feel when things are near them
• Bugscope TeamThey use the hairs to sense their environment...although technically, spiders are not bugs, or insects ;)
• Bugscope Teamthese are simple eyes, and spiders have 8 of them
• Bugscope Teamsome spiders see better than others, but even with eight eyes they often do not see well
• 10:28 am
• Teacherare there any presets with a good view of a compound eye?
  Bugscope Teampreset 6 shows a nice on on a leafcutter ant
• Bugscope Teamyeah now that you mention it

• Bugscope Teamthere is also preset8 which is the mosquito eye
• Teacherwhat are those pores on the spider

• Bugscope Teamthis is a cool view -- the things that resemble pores we think are sockets for missing hairs

• Bugscope Teamsome spiders, like tarantulas, have itchy 'urticating hairs' that they release to prevent curious animals from bothering them
• Teacherturning over the camera to another student

• Bugscope Teamhere are some ommatidia for you
• Bugscope Teamthis is on the mosquito compound eye
• Bugscope Teamthese are a little shrunken

• Bugscope Teamthey are a little deflated from what they would normally look like, so they look like beanbags
• Bugscope Teamif you decrease the mag you will see where they are

• Bugscope Teamnow you can see the whole right eye, almost
• Bugscope Teamand the base of the antenna
• 10:34 am
• Bugscope Teamand you can see that mosquitos have scales like moths, butterflies, skippers, silverfish, and few other insects
• Bugscope Teamif you click to drive remember to click to stop

• Bugscope Teamthis is carbon tape

• Bugscope Teamthe doublestick tape Cate used to mount the samples upon
• Bugscope Teamwe stick the insects on double stick carbon tape to ground the charge, and the carbon tape is stuck on to an aluminum disk

• Bugscope Teamanother eye!

• Bugscope Teamyou may want to avoid sending the microscope any more commands until it slows down

• Bugscope Teamuntil it stops in one place

• Bugscope Teamyou are driving a $600,000 microscope from your classroom
• TeacherHow are we doing?
• Bugscope Teamtry a preset
• Bugscope Teamto get back on track

• 10:39 am
• Bugscope Teamhere is a rolypoly your class sent

• Bugscope Teamphew!
• TeacherThis is Mr. Kelley again. What are we doing wrong?
• Bugscope Teamyou might be double-clicking on the presets, when it only requires one click, which would cause it to drive erratically
• TeacherGotcha. Single click for presets.

• TeacherWe promise only a single click this time. Can we try anything else?

• 10:44 am
• TeacherAre we back on track?

• Bugscope Teamokay I think so...

• TeacherWhat are we looking at now?
• Bugscope TeamAlright, do you see the jaws, Mr K?
• Bugscope Teami got us to the jaws on the centipede you sent us
• Bugscope Teamnow try taking the mag down, one click
• TeacherVery neat. I am the only one driving now, so as to prevent confusion.

• Bugscope Teamnice
• Bugscope TeamI think we are back on track
• TeacherEverything is looking good from our end now.
• Bugscope Teamgo ahead a cruise around, just a click at a time
• Bugscope Teamand = a
• TeacherOkay. Sorry about the double-clicks!
• Bugscope Teamif you take the mag down from here you should be able to see the head of the centipede
• Bugscope Teamno problem, everything is fine now

• Bugscope Teamoh!!!
• Bugscope Teamthe two little balls in the middle right are pollen grains

• Bugscope Team the pollen grains got covered with juju
• TeacherVery neat!

• 10:50 am
• TeacherTrying to focus on pollen grains.

• Bugscope TeamMr K since we made the preset, an hour ago, some hemolymph oozed out of the cuticle of the assassin bug and obscured the pollen grains
• TeacherUh oh. Occupational hazard!
• TeacherWe'll try another preset.
• Bugscope Teamthey are the juggernaut-like things in the upper right, but they don't look so good anymore
• Bugscope Teamcool

• Bugscope Teamthis is a spiracle, which is a breathing hole- kind of like our nostrils
• Bugscope Teamit even has little hairs in it to help keep unwanted stuff out
• TeacherYou read our minds!
• Bugscope Teamthere are usually two spiracles per segment, on either side of the body

• Bugscope Teamthough i dont think they sneeze, that would be funny to see- a little insect sneezing
• Bugscope Teamwe read our own minds as well -- happens sometimes

• Teacherare these the little hairs? similar to our cilia?
  Bugscope Teamyes that's right
• Bugscope Teamhere we see some charging, where the sample is not adequately coated with gold-palladium in some places
• Bugscope Teambut this is great detail
• Teachercan we magnify more?
• Bugscope Teamtry it
• Bugscope Teamgo ahead and try, i don't see why now
• Bugscope Teamnot*
• Bugscope Teamno charging right in the middle

• 10:55 am
• Bugscope Teamyou can see the micron bar on the lower left of the image
• TeacherThis is excellent!

• Bugscope Teama bacillus bacterium is usually 2 microns long
• Bugscope Teamlike E coli or anthrax bacteria
• Bugscope Teamso you can see that the cilia we are looking at are close to the size of bacteria
• TeacherGreat info. We are discussing prokaryotic vs. eukaryotic cells. The size reference was helpful.
• Bugscope Teamif there were bacteria on these critters we would have no problem seeing them
• TeacherHeaded for another preset.
• Bugscope Teamcool

• Bugscope Teamspider claw, spider claw
• Bugscope Teamthis is a spider claw, i tried to get the best one we could find

• Bugscope Teamyou can see the plumose setae around it
• Bugscope Teamsome spider silk is not sticky
• Teachersetae surrounding claw?

• Bugscope Teamalso, spiders are good recyclers -- they eat their web when they are done with it
• Bugscope Teamthere is a lot of setae around spiders, so they are really attuned to vibrations

• Bugscope Teamspider web is a good source of protein for them when they are hungry
• Bugscope Teamyes setae all around the claw, which is built like a comb

• Bugscope Teamnice detail here as well
• 11:00 am
• Teacherexcellent detail!
• TeacherThe fibrous detail is unbelievable!
• Bugscope Teamthe claws are hardened, kind of like insect cuticle, or chitin, which is like our fingernails
• Bugscope Teambut spiders have soft bodies, and when they air dry, after they die, most of the body will shrivel
• Teacherheaded for another preset.
• Bugscope TeamCool!

• Bugscope Teamthis is beautiful
• Teacherspiracles or empty hair "follicles?"
• Bugscope Teamwe are not sure just what we are seeing -- whether there is a function here beyond ornamentation
• Bugscope Teamwe think those are empty 'follicles,' as you said
• Teacherso the patterns (dark areas) do not have the ridges?
• 11:05 am
• Bugscope Teamthis pattern of smooth areas would look kind of like racing stripes at a lower mag view, like if you were another spider
• Bugscope Teamyeah!
• Bugscope Teamsometimes we see placoid sensilla on antennae, for example, that resemble the dark areas, but this is a little different
• Bugscope Teamwe have never seen this before, that I recall
• TeacherInteresting! Well the bell just rang, so it is just me now.
• Bugscope Teamplacoid sensilla are sensory organelles on the antennae or sometimes on limbs that may help the insect/arthropod sense heat, for example
• TeacherThis has really been a treat. Sorry abou the hiccups early on in the session.
• Bugscope Teamif you have time just cruise around -- it will add images to your class's database for later viewing
• Bugscope Teamno problem, sorry that happened to you at such a bad time
• TeacherThank you I will do that and perhaps round up some other teachers.
• Bugscope Teamwe want you to see the leafcutter ant, which we may take high res images of later
• Bugscope Teamcool\
• 11:11 am

• Bugscope TeamYay!
• Bugscope TeamThis is my favorite.
• TeacherThis is amazing. Was this one of our samples?
• Bugscope Teamthis is something we got from Annie, our entomologist, who had to get back to work. She was on earlier.

• TeacherAmazing find by Annie!
• Bugscope Teamit is unusual, in our experience, to see serrated jaws like that
• Bugscope Teamleafcutter ants are very cool looking with their muscular heads and their long powerful jaws
• TeacherI am going to try to get a better look at those jaws.
• Bugscope TeamAnnie gave us a small vial of them in ethanol, and we critical point dried them

• Bugscope Teamyes as Cate says much of the inside of the head is muscles that control those badboy jaws

• Bugscope Teamyou can use click to center at a range such as this
• Teachermissed 'em!

• Bugscope Teamwhen you activate click to center, rather than driving, you can click on the screen with your mouse, and the 'scope will drive to that position
• 11:16 am
• TeacherUnderstood.

• Bugscope TeamNice!

• TeacherAmazing.
• Bugscope Teamsort of like pinking shears

• Teachergonna take a look at that compound eye on the left.

• Bugscope Teamants often have very few ommatidia in their compound eyes, and some ant species do not bother to have eyes at all -- they rely on their antennae to get information
• Teacherwould this be considered very few?

• Bugscope Teamthis is kind of medium -- some have like 10 or 12, it seems

• Bugscope Teamsome ants have compound eyes with many facets, like a wasp, which they are related to
• 11:22 am
• Teacherlooking for some good ole bacilli, spirilli, or cocci on this ommatidia!

• Bugscope Teamin our experience, terrestrial insects have less complex eyes than flying insects

• Bugscope Teamwe usually see bacilli, when we do find them
• Bugscope Teamif we were to see spirochaetes I would be surprised. they are bad little dudes
• TeacherInteresting point about terrestrial vs. flying.

• TeacherUnbelievable!
• Bugscope Teamflying insects sometimes have the ability to see in the UV as well

• Bugscope TeamI am sure there are terrestrial insects that can see UV but I don't know which
• Bugscope Teamthis is cool

• Bugscope Teamthis is the part that pokes into you of the mosquito
• Teacherhave another science teacher here now. we are like kids in a candy store.

• 11:27 am
• Bugscope Teamusually we never see any of them -- they're all inside, closed appressed, as they are often described
• TeacherDo they use one or more when they poke?

• Bugscope Teamthese mosquitos were critical point dried, and the individual components of the biting/cutting/bloodsucking mechanism are exposed
• Bugscope Teamthey use them all, in a side by side cutting motion
• Bugscope Teamthey use them all to help dig into whatever body part they are trying to suck blood from.
• Bugscope Teamlike laciniae, with a flea
• TeacherDo these ridges cause the stylet to stay in you skin? They run in the opposite direction for exit.

• Bugscope TeamI think it would help, having recruved spines, like we see with ticks
• Bugscope Teambut they are very small, and they move back and forth, so I imagine their main purpose is to cut efficiently

• Teachermy colleague wanted to see the moth scales.
• Bugscope Teamrecurved, sorry can'tt ype.

• Bugscope Teammoth scales are hard to image because they are usually isolated from ground -- they charge up with electrons
• Teacherlost my image.

• 11:32 am
• Bugscope Teamhit refresh
• Bugscope Teamdid that work?
• Teacherback on track.
• Bugscope Teamthis is cool because we really cant see these with our eyes, they just look like powder
• Bugscope Teamscales can be responsible for structural colors -- colors that are due to the shape and spacing of the ridges rather than pigments
• Bugscope Teamwe have a couple of butterfly scale images in a French-Australian documentary on color called something like Breaking the Colour Code.
• Teacherwhat is the difference between the smaller ones and the large on in the center, other than size?
• Teacherone in the center, sorry.
• Bugscope Teamwell they come from different places, not really sure
• Bugscope Teamthe one in the center has probably fallen there
• Bugscope Teamscales are helpful if, for example, you are flying along and run into a spider web
• TeacherInteresting that lareger one is not lobed like the smaller ones.
• Bugscope Teamyou can slip out and leave the scales
• Bugscope Teamthey also have different kinds of scales, like you can see the more wispy ones coming in from around the edges
• Bugscope Teamsilverfish have scaels too
• Bugscope Teamscales
• Bugscope Teamskraelings

• 11:37 am

• Bugscope Teamnice imaging

• Bugscope Teamthink of how the grooves in a record, of black vinyl, sometimes refract colors

• Bugscope Teamthe ridges in the scales refract color

• Teacherdo i need to focus?
  Bugscope Teamyes a little bit it looks like
• Bugscope Teamyes if you can
• Bugscope Teamtry it
• Bugscope Teamif it gets worse go the other way
• Bugscope Teamcoming in nicely

• Bugscope Teamwhen we run -- nice -- bugscope we put the samples at a long working distance so the kids can see low mag images of the critters
• Bugscope Teamthat is very good
• Teacherare the scales actually hollow or is the black the refraction?
• Bugscope Teamat long working distances we do not get the best resolution
• Bugscope Teamthis is phenomenal, very good
• Bugscope Teamthey are hollow
• 11:42 am
• Bugscope Teamthe refraction occurs with light, and we are using electrons rather than light
• Teachercool. I may change the saying to "light as a hollow moth scale!"
• Bugscope Teamwe do see optical effects with electrons, sometimes
• Bugscope Teamthey are hollow to keep the weight of the scales down and also they are probably used to help give lift to the wings
• Bugscope Teamyou have done a great piece of imaging here
• TeacherThank you. Let me know when my time is up.

• Bugscope Teamwhen we can we add an hour to the end of a session in case there are problems

• Bugscope Teamso we have a few more minutes...
• Bugscope Teamthis is cool, Chad -- double claws
• Teacherthank you. I just to make sure I take a look at all of the presets for the database. I lost track at the beginning because of the connection problems.

• Bugscope Teamso whenever you want you can visit your chat and images at your member page http://bugscope.beckman.uiuc.edu/members/2008-134/

• TeacherThank you. Do we have some lint on this claw?
• 11:47 am

• Bugscope Teamlooks like plant matter

• Bugscope Teamyeah sorry the sample was a little dirty
• Teacherjust kidding. it is extraordinary.

• Bugscope Teamcellulose

• Bugscope TeamChad be sure and sign up again

• Bugscope Teamyou can try to focus if you want

• Bugscope Teamnice

• Teacherthat cellulose is just perched atop that claw!

• Bugscope TeamChad and Karen I am going to peel out, try to beat the nerds to the cafeteria. Thank you for connecting today, for sticking with it. This was a very good session.

• Bugscope TeamCate can take it from here; see you next time!
• TeacherThank you everyone! This was great.

• Guest I enjoyed it!
• 11:52 am

• Bugscope Teamwe like it when people have fun doing this, we have fun as well-- it doesn't quite seem like work
• TeacherOkay I will tear myself away. I will be submitting our feedback very soon. Thank you again!
• Bugscope Teamthank you for joining us
• Bugscope Teamgoing to bring down the session