Connected on 2013-01-11 13:30:00
from Gallatin, Montana, United States
- 12:21 pm
- Bugscope Teamcoating sample...
- 12:32 pm
- Bugscope Teamsample is now pumping down
- Bugscope Teamhello Mr McG!
- TeacherWe will have our first group coming in around an hour. Just logging on to test.
- Bugscope Teamsuper cool. Once the sample pumps down we'll start making presets, should be good on timing.
- 12:50 pm
- 12:55 pm
- 1:01 pm
- Bugscope Teamfleurs du mal
- 1:07 pm
- 1:13 pm
- 1:19 pm
- 1:24 pm
- TeacherOK - we are getting kids logged in
- Teacherlet me know if you are ready for us
- Bugscope Teamwe just have to get the cricket and we're done
- Bugscope Teamok we are good to go!
- Bugscope Teamyou should have control of the 'scope now
- 1:30 pm
- Bugscope Teamplease let us know when you have questions about anything
- Bugscope TeamHello Everyone!
- Bugscope TeamWelcome to Bugscope!
- Bugscope Teamthis is a rolypoly
- Bugscope Teamyou can barely see its eye, at least one of them
- Bugscope TeamMr McG is the Supreme Ruler of the microscope
- Bugscope Teambut here, let's go to the eye
- Teacherdoesn't look like I have a dashboard - presets not moving and no zoom functions
- Bugscope Teamhuh what about now?
- Bugscope Teamsorry
- Bugscope Teamyou should have control now
- Bugscope Teamyou can see the individual facets of the eye
- Bugscope Teamonly about 10 ommatidia
- 1:35 pm
- Teachertried logging out and back in but still no luck. I'll try another browser
- Bugscope Teamwait we gave you control as Mr M
- TeacherOK - now it is working!!!!
- Bugscope Teamsuper cool
- Bugscope Teamthose are setae that help the spider sense what is near it or touching it
- Studentwhat are the hairs on the fangs?
Bugscope Teamthose are hairs. They help the spider and insects that the hairs are on to feel what is going on around it
- Bugscope Teamwe call the hairs setae when they are on insects and spiders
- Studentis it a venomous spider?
Bugscope Teamall spiders are venomous, but some are dangerous to people
- Bugscope Teamthis one is not dangerous to people, particularly
- StudentWhat type of spider is it?
- 1:40 pm
- Bugscope TeamI just heard the stage click (I'm sitting at the microscope -- the SEM). Sometimes when we hear that click it means that the stage is bound up, at least a little, and the presets may not work perfectly
- Studentdo spiders have mucles
Bugscope Teamyes they do, on the insides of their bodies like we do
- Studentwhy do the spiders have small hairs
Bugscope Teamthey need to be particularly sensitive to vibration, and those hairs help them sense sound and touch and other vibrations, even if they do not have webs
- Studenthow much does a electron microscope cost?
Bugscope Teamaround $600,000. we bought this one is 1998.
- Studenthow big is the electron microscope?
Bugscope Teamit is about the size of a large desk, with a side cabinet, and also a part with the stage on it that is about 6 feet tall
- Bugscope Teamoh also we do not know what kind of spider it is, I am sorrry
- Studentreal name
- Studentwhat is a roly polys rear name?
Bugscope Teamsometimes they are called pill bugs, or sow bugs, or wood louse
- StudentHow many legs does a rolly polly have
Bugscope Teamthey have 7 pairs of legs
- Bugscope Teamone of the genuses of rolypolies is called Armadillium. You can guess why...
- Studenthow far can we zoom in
Bugscope Teamthis microscope can zoom up to a million times, but you probably won't see much above 40,000 times for these bugs
- Bugscope Teamthose are setae
Bugscope Teamsome are microsetae, which do not connect to nerves, through the exoskeleton, and some are setae, which do
- 1:45 pm
- Studentwhat is the string like thing?
Bugscope Teamthat is fungus -=- the strands are called fungal hyphae
- Studentwhat is the most interesting bug that you know
Bugscope TeamI really like leafhoppers, and I like weevils; springtails are interesting and they may not actually be insects
- Studentcan the microscope see atoms
Bugscope Teamno, not this one. We can see them using the atomic force microscope (AFM)
- Studentwhat are microns
Bugscope Teama micron is the same as micrometer, which is a thousandth of a millimeter or a millionth (that is what micro- means, in this case) of a meter
- Bugscope Team2 microns is the length of most rod-shaped bacteria (the bacilli)
- StudentWhat is your favorite experiment you've ever conducted with the electron microscope?
Bugscope Teamwe did this really cool thing called mite wars a few years ago; the goal was to let a bunch of mites that attacked other mites go after mites that eat plants; it was kind of wild
- 1:51 pm
- Studentwhat wasp has the longest stinger and how long is it
Bugscope Teamsome of the cicada killer hornets have stingers that are several centimeters long
- Studentwhy was the stinger not pointy?
Bugscope Teamit was somehow bent, I am not sure why.
Bugscope Teamnot really sure, maybe it helps to sting other insects. It does look like it would hurt a lot
- Studentwhy are there hairs on the black part but not the white part
Bugscope Teammaybe we can just see them better against the black areas
- Studentdo bees sting with the same venom
Bugscope Teamyes, but it is usually more concentrated
- TeacherWhat are we looking at here guys - the FSM baby?
Bugscope Teamthis is the click region of the click beetle, and the thing we see that looks like an octopus is something we see fairly frequently; it seems to be a plant that flies through the air; but we were thinking it looked like a flying spaghetti monster
- 1:56 pm
- Bugscope Teamthis is some bacterial plaque -- the rod-shaped structures, yes
- Teacherwhich are the bacteria - the small rod shaped structures?
Bugscope Teamthat would be exactly right
- Studentwe brush our teeth and they obviously dont have toothbrushes so can they drowned in there own bacteria if enough grown in there mouth?
- Studentwhy is it smiling
Bugscope Teamhaha. that is the curve of the mandibles
- Bugscope Teamhere you can see the compound eye up close
- Bugscope Teamthe mandibles open side to side, like a gate
- Bugscope Teamyeah this could be focused....
- Bugscope Teamthe individual facets of the compound eye are called ommatidia
- 2:01 pm
- Studenthow many of these circles are on this eye?
Bugscope Teamprobably several thousand per compound eye
- Bugscope Teamwe can see that it has some dried salts on it
- Bugscope Teamthat is what those little crystal shapes are
- Teachernice job focusing!
- Bugscope Teamthis eye has fewer ommatidia
- Studentwhat is the eye made out off and why do people call others bugeyed
Bugscope Teaminsects usually have big bulbous compound eyes that usually stick out from the rest of their head. The compound eye is made up of the same stuff as the rest of the insect exoskeleton. People may be called bug-eyed because they have big eyes or are able to kind of pop their eyes out a little.
- Bugscope Teamit has a few of those gnarly looking fungal hyphae on it
- Bugscope Teamif you had compound eyes you would have better peripheral vision, and also -- you would have a better ability to see motion, which is a good thing in the insect world
- TeacherLike Marty Feldmon
Bugscope Teamyeah exactically
- 2:07 pm
- Studentwhere are you guys at un relation to the microscope?
Bugscope Teampresently I am sitting at the microscope and using its computer to talk with you; Cate is upstairs in her office, but she made the sample and was down here to help set up; Scot is another computer down here, and sj is me in my office
- Studentwhat kind of light do earwigs see? like bees see ultra violet rays?
Bugscope TeamI don't know for sure, but I imagine earwigs do not see in UV and their vision is fairly basic
- Bugscope Teambees so some colors well and others not so well at all; I believe most of them can also see in the UV but I am not sure
- StudentWhat are we looking at here?
Bugscope Teamthis is the end of one of the beetle palps. they help the beetle taste food and to move the food around in its mouth
- Studentwhat is a palp?
Bugscope Teama palp is a kind of accessory mouthpart that is like a limb, like a knife and fork that can also taste
- Studentthank you!!!
- StudentTHSNK U GOT TO GO
- StudentThank you go to go!
- Studentthank you very much!!!!!
- Studentthank you for this lesson!
- Bugscope Teamthanks!
- StudentThank You for your time
- Teachera bit of a break and then one more group coming in, about 5-10 minutes
- Bugscope Teamum that below was supposed to say Bees see some colors well...
- Bugscope TeamThank you, Everyone!
- Studentthanks for showing us these images and what a great lesson
- Studentthank you!!!!
Bugscope TeamThank you, Dax!
- Bugscope TeamBye you all!
- 2:12 pm
- Bugscope Teamhello Dragonfly! Are you still with us?
- Bugscope Teambees are said to not see red very well at all; they can actually confuse it with green. it is also said that if they were not able to see a UV wavelengths, they would lose interest in foraging
- Bugscope Teamhttp://www.westmtnapiary.com/Bees_and_color.html
Bugscope TeamHi TrevyM!
Bugscope Teamhaha Nice.
- Bugscope Teamthis is a beetle; you can see it has lamellated antennae
- Bugscope Teamthe antennae have lobes and can actually fold open kind of like a fan
- 2:18 pm
- Bugscope Teamyou can see the palps -- two sets of palps. and yo can also see one of the compound eyes
- Bugscope Teaminsects and comparable arthropods like centipedes and rolypolies do not have skin or bones; they have an exoskeleton instead, which is kind of like if you were wearing a suit of armor
- Studentcan we see the silverfish head?
Bugscope TeamI just clicked on it, hope Mr McG is cool with that.
- Studenthow big is it
Bugscope Teamwe can get an idea of the sizes of what we're looking at by referring to the scalebar on the lower left of the screen. you can see that it reads in millimeters right now
- Bugscope Teamthe silverfish's head is sideways
- Studentwhat is the furry stuff on it
Bugscope Teamwe see lots of setae, which look like hair, and we also see lots of scales, which are actually also setae, and which butterflies, moths, and mosquitoes have as well\
- 2:23 pm
- Student b
- StudentHow big is the microscope
Bugscope Teamit is kind of like a large desk with a taller component to one side, about 6 feet high, and also a cabinet toward the back. it has its own room, air, nitrogen, filtered electrical power, chilled water, etc.
- StudentQUE FIGURA ES
Bugscope Teamthis right now is the tip of the stinger of a small wasp
- Bugscope Teamif you go to the Bugscope web site when you're done, you can see a photo of the microscope
- StudentWhat is the moon crater looking thing on the stinger?
- Bugscope Teamwe can see one of the wasp's arms to the left. it is glowing because it is charged up with electrons from the electron beam we are using to collect these images
- Studentwhy does it hurt when the wasp stings you
- Bugscope Teamwe coat the samples with gold-palladium alloy to make them conductive, but sometimes the connection to ground is lost and we see evidence of charging like that
- Studentwhat are the bumps on the wasp
Bugscope Teamthe wasp has some sort of dried liquid on it. That's what the bumps on the wasp are. The big bumps under it are features of the double stick carbon tape
- Bugscope Teamwe are sorry this is a little difficult to see; I can work on it a bit, at the microscope, if you have time
- Teacherplease do
- 2:28 pm
- StudentPlease do
- Studentthats bacteria?
Bugscope Teamthe rounded rod-shaped things are bacilli -- one of the three general shapes of bacteria
- Studentare they still alive
Bugscope Teamyeah they are kind of like stubby bananas, like plantains
- Bugscope Teamthe beetle had a dirty mouth; we are looking at the mouth now
- Studentwhat is tes
Bugscope Teamthis is the mouth area of the beetle
- Bugscope Teamnow we can see some of the palps
- Studentis this black and white or is it a different color
Bugscope Teamit's in grayscale (black and white). To get those bacteria and virus images in color we have to do that after we take the image
- Bugscope Teamand two of the bacteria
- Studentthat looked like a pancake
- Studentwhat type beetle is this
Bugscope Teamnot sure; it is kind of like a june bug and kind of like a Japanese beetle, but it is neither
- Bugscope Teamthere are more beetle species than any other insect species
- 2:34 pm
- Studentwhat type of spider is it
Bugscope TeamNicken this is some kind of house spider
- StudentWhy are some senttae longer then others?
Bugscope Teamthe bigger ones probably help tell the spider it is bumping into something- like cat whiskers.
- Studentwhat is the fuzz in the background
Bugscope Teamthe double-stick carbon tab that the insects/arthropods are stuck to is in the background, and it has bumps on it. some of those bumps are due to silver paint as well
- Studentwhy is the spider furry
Bugscope Teama lot of the hairs probably help the spider feel vibrations (like from prey)
- Studenthow are spider
- Studentcoating sample plis
Bugscope Teamthe coating was done with an alloy of gold and palladium, using a sputter coater, which can produce a very thin layer of conductive metal
- Studenthow come tarantulas don`t make webs
Bugscope Teamthey don't need to, apparently, but they do produce the same silk
- Studentis that the mouth?
Bugscope Teamyes, the beetle's mouth right now
- Bugscope Teamthe thing that goes down and curves around the mouth like a club is an antenna
- Bugscope Teamtarantulas are very fragile. some of the hairs we see on tarantulas are called urticating hairs, which means that they induce itching in whatever comes close enough to sniff them
- StudentWhere did you get all these insects?
Bugscope Teamwe collect them ourselves, we get them from entomologists, people send them to us
- 2:39 pm
- StudentHow many legs does it have?
Bugscope Teamthey have 14 legs
- Studentwhat are those things rolled up on top of its head
Bugscope Teamthose are the antennae
- Studentwhat is the scientific name for a roly-poly
Bugscope Teamone of the rolypoly genus names is Armadillium, so a particular species would be Armadillium something.
- Studenthow do they get they're legs inside themselves
Bugscope Teamthey kind of fold up
- StudentDoes the roly poly have eyes?
Bugscope Teamyes it does; let us know if you want us to take you there to see them
- StudentW.H.A is der
- StudentWhy are there holes in the rolly pollys legs?
Bugscope Teamthere are places where the legs broke off. Insects can break easily after they die and dry out
- Studentyes please!
Bugscope TeamBolivia here is one of the compound eyes; you can see that it has about 10 or 12 facets, or ommatidia. Some large wasps can have as many as 17,000 ommatidia per compound eye.
- GuestI'm the defender of the free world
Bugscope TeamThanks, man.
- Studentdo roly poly's grow their legs back
Bugscope Teamno. the only way a bug would grow something back is if it had another molt to go through. Some spiders that live a long time like tarantulas will have multiple molts. If a caterpillar lost a leg it would have all of its legs when it turns into a butterfly or moth
- Studenthi obama
- Studentwhy does it have weird eyes
Bugscope Teamthey probably do not see very well; mostly they would just register light and shadows
- Studentwhats that bumpy thing?
Bugscope Teamthat is the compound eye
- Studentwhat is the clump on its eye thats connected to the sting stuff?
Bugscope Teamsome juju -- dried fluid and dirt
- StudentYIJICA its the eye. :)
- 2:44 pm
Bugscope Teamreally not sure; we may be seeing some fungal hyphae, or it could be web from a spider
- Bugscope Teamthe President was not here for very long
- StudentAre the claws sharp?
Bugscope Teamyes but they are so small you would hardly feel them
- Studentthere settae on claws?
Bugscope Teamthere are some. You have to remember the insect really can't feel much through the exoskeleton
- StudentWhy are they hooked together?'
Bugscope Teamit just happened, apparently, when the beetle died. we do not see that very often
- Studentwhat is dis because i don not
Bugscope Teamthis is the abdomen of a beetle, and the tarsi of the hindlegs. tarsi are the last five segments of the leg.
- StudentCan u see these claws with the naked eye?
Bugscope TeamI think just barely.
- StudentHarry spider knee time!
Bugscope Teamyes it is
- Studentis he sharping his claw
Bugscope Teamno, probably doesn't need to
- 2:49 pm
- Bugscope Teamspiders have, among other types of setae, plumose setae, which means that they have projections all of the way around, kind of like a Christmas tree.
- StudentDo u know how old the spider was?
Bugscope Teamno I am sorry
- Studentwhat are the things that look like corn flakes
- Bugscope Teamthe things that might look like rocks right now are most likely dust and dirt
- Studentwhy do they need septa in there mouth
Bugscope Teamit's like a filter device
- Studenthow much did the microscope cost
Bugscope Teamthis one was about $600,000 in 1998, and recently we checked into getting a new one -- they cost about the same
- 2:54 pm
- Studentare the setae sharp?
Bugscope Teamyes they are, but from our perspective they are so small that they would only tickle if you could feel them
- Studentgross. :(
Bugscope TeamBolivia that is part of what makes this fun!
- Bugscope Teamif you were holding a ladybug you might feel some tickly feeling from the claws. Not so much if it bit you though
Bugscope Teamyes you would definitely feel it if it bit you
- Studentwhere did you find these arthopods
Bugscope Teampeople send them to us, bring them in in jars, we collect them in the summer, sometimes in our own houses. it is cool because when we come across bugs we are the predators and can actually make use of them
- Studentwhen cool kids like us arent using the microsope who is
- StudentWhat other jobs do you guys have?
Bugscope Teamwe help other people image their samples for their research. We might also image samples for companies. We don't just focus on insects
- StudentWhen you are not using the microscope who is?
Bugscope Teamgraduate students and postdoctoral scholars, so people who are done with college, and sometimes college studetns
- Studenti saw some yellow stuff come out of a lady bug is that its blood
Bugscope Teamit could be or it could be bug waste
- Studentwhat are your backgrounds,cate/scot
Bugscope TeamI have a degree in English and biology; Cate was a physics major, so she is smart
- Studentnice beetle leg
- StudentTHANK YOU
- Bugscope Teamthe hemolymph (bug blood) is usually clear, but when you mash a bug you sometimes get other stuff mixed in
- Studentthank you very much
- Studentthank you
- StudentTHANK YOU SOOO MUCH!!
- Studentthank you !!!!!!!!!!!!!!!!!
- StudentThhhhhhhaaaaannnnkkkk uuuuuuuuuuu! had a great time!
- StudentTHX :)
- Bugscope Teamthank you!
- 2:59 pm
- StudentThank you so much scotj and cate!!!
- StudentTHANK YOU PEOPLE!
- Studentthank you: :) bye
- Student ]
- StudentPeace homeis!
- Bugscope TeamThank You!
- Bugscope TeamThank you, Bolivia!
- StudentThank u for letting us use the microscope!
Bugscope TeamThank you, Montana.
Bugscope TeamBye LoveMichelle!
- StudentGracias! :):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)
Bugscope TeamGracias, Todd!
- Studentthank you :):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):):))::):):
Bugscope TeamThank you, Drab & Hanley!
- TeacherAwesome job team. Thank you so much for hosting us. The kids loved it!
Bugscope TeamSee you next year!
- Studentthank you, from dylan and noah
Bugscope TeamThank you, Dylan and Noah!