Connected on 2011-11-15 09:30:00
from Gallatin, Montana, United States
- 8:26 am
- Bugscope Teamwe will start making presets in a few minutes
- 8:39 am
- Bugscope TeamGood morning, Jerry!
- 8:45 am
- 8:50 am
- Bugscope Teamwe're making the presets for today's Bugscope session, which starts at 9:30 central time
- 8:58 am
- 9:03 am
- 9:08 am
- Bugscope TeamGood morning, Mr McGeehan!
- Bugscope TeamWelcome back to Bugscope!
- TeacherHi guys. Brian McGeehan here. I'll be bringing kids in around 9:25 to have them start logging in. Looking forward to it. We have two classes back to back - will be about 10 minutes in between
- Bugscope TeamCool we are working to finish up the presets.
- 9:15 am
- TeacherBack in about 10 or 15 - time to round up the kids.
- 9:21 am
- 9:26 am
- Bugscope Teambrb
- Bugscope Teamalright we are ready to roll!
- 9:31 am
- TeacherOK...back - kids are just coming in - it will take 2-3 minutes for them to log in
- Bugscope TeamSounds great!
- Bugscope Teamthis is the head of a female housefly
- Bugscope Teamshe's sticking her forearm out toward us
- Bugscope Teamgood morning LanceNate!
- Bugscope Teamgood morning, everyone! I cannot type all of your names that fast.
- Bugscope Teamplease let us know when you have questions
- Bugscope TeamI am at the SEM and can drive the 'scope directly when it's necessary.
- Bugscope Teamthe presets, from which whomever is driving may select, are on the screen to the left
- 9:36 am
- Bugscope TeamHi Jordan!
- Bugscope TeamWelcome to Bugscope!
- Studentu have a ps3
Bugscope Teamno we just play with equipment like this
- Studentis the Japanese beetle poisinous
Bugscope Teamno I don't think so but it is likely not tasty
Bugscope TeamHi Garrett!
- Bugscope Teamthis is the third set of legs
- Studenthey jamie and shelby
- Studentit looks so cool
Bugscope Teamwe are lucky to have this
- Bugscope Teamthe last several segments of an insect's leg are the tarsi, including the segment with the claws, if it has claws
- Bugscope Team'mudra' is a joke -- it's what the position of a Buddha's is called
- StudentIs this the highest magnification microscope in the world? How high is the magnification?
Bugscope Teamwith a transmission electron microscope it is possible to see atoms
- Bugscope Teamthis is relatively low magnification but very good resolution
- Studentwhat is this bug????????
- Studentwhat is this bug\
Bugscope Teamthis is a Japanese beetle
- 9:41 am
- Bugscope Teamit is a plant pest
- StudentHow many people are usually at the microscope?
Bugscope Teamusually one person uses the 'scope at a time, to do her/his own research
- Bugscope Teamfor Bugscope we usually have one or two people
- Bugscope Teamto try and answer questions
- Studentso what are the hairs on the bug for?
- Bugscope Teamthey are sensory, usually
- Bugscope Teambecause insects do not have skin, they have tiny hairs called 'setae' that stick through the cuticle and help them sense their environment
- Studentwhy are there hairs inside a ant mouth
Bugscope Teamto help the ant taste and manipulate its food
- Bugscope Teamthe ant has two mandibular and two maxillary palps that are like tiny accessory limbs
- Bugscope Teamin its mouth
- Bugscope Teamthis is the compound eye
- StudentHow many circles does an ant have in it's eye?
- Studentdo ants have teeth? if so, how many?
Bugscope Teamno insects have real teeth; the ant has two mandibles that open from side to side like a gate, and the mandibles (jaws) have serrations on them that resemble teeth
- 9:46 am
- Bugscope Teamsometimes the mandibles are hardened at the tips -- they have metal in them, such as zinc, to make them harder
- Bugscope Teamwhen an ant's mandibles become worn, it may retire from what it is doing, using its mandibles, and do something else
- StudentWhat is a spiricle?
- Teacherlooking for spiracles now
Bugscope Teamwe made a preset of a spiracle on the body of the stinkbug
- StudentWhat is the eye called beside a compound eye?
Bugscope Teamthe individual facets of the eye are called 'ommatidia,' singular is 'ommatidium.'
- Bugscope Teamtiny pores like this are sometimes tentorial pits
- Studentdo ants have eyelids?
Bugscope Teamno they do not have anything to cover their eyes, but they do not really sleep either
- Studentis this a spiracle
Bugscope TeamI think it is a pit that does not go anywhere. On the inside there will be an extension of the cuticle that the muscles are attached to.
- Studenthow many compound eyes in one eye of an ant?????????
Bugscope Teamit depends, of course, but usually it is a small number. There can be from around 12 to 1000.
- Bugscope Teamsome ants do not have eyes at all
- 9:52 am
- Studentwhen does a stinkbug spray? when it's angry or scared?
Bugscope Teamyes that is exactly right
- Bugscope Teamyou can see the external opening of the sink gland on the other side of the body
- Bugscope Teamnow you can see the tip of the proboscis
- Bugscope Teamstinkbugs are 'true bugs' -- hemiptera
- Studentis it both or is it just one?
- TeacherI think Jake and Gavin ment entomologist
- Studentwhat is the stuff that makes up the stink bugs spray
- Studentscot are you an arthoplagyst
Bugscope TeamI am an entomologist only by default, that is, not really. I do hang around with them and ask a lot of questions.
- Bugscope Teambecause I have been doing this for more than 12 years I have learned a few things. I have a degree in biology and english and have been doing electron microscopy for a long time
- Studentis this a sucking mouth?
Bugscope Teamit is a piercing/sucking mouth
- Studentis it a chopping mouth? what do they eat??
Bugscope Teamthey are plant pests, so they poke their proboscises into leaves and stems and branches and suck the juices out of plants
- 9:57 am
- StudentIs this a sucking mouth
Bugscope Teamyes it is -- piercing sucking mouthparts
- Studentwhat do they pierce and what do they suck up?
Bugscope Teamleaves, stems, small branches; they consume the plant fluids
- StudentWhat are polen grains made of?
Bugscope Teamthey are made of a number of proteins, some of which apparently people cannot agree upon
- Studenton the polen do the divets serve any purpose?
Bugscope Teamthey help it stick to things and thus make the pollen mobile or suited to being carried around
- Studentis the moth eye different from the ant eye?
Bugscope Teamyes it is better, and especially at seeing in the UV
- Studentwhat are the feathery things on the moth?
Bugscope Teamthose are scales, which are modified setae
- Bugscope Teamif you were a butterfly or moth or mosquito or silverfish, your scales would help protect you when you might fly into a spider web
- 10:02 am
- StudentHow long have you been running the Microscope? Months? Years?
Bugscope TeamI have been doing electron microscope full time since May 1983.
- Studentthis is really cool how many other schools get to do this in a years time???
Bugscope Teamprobably 40 to 45
- StudentWhat is the UV?
Bugscope Teamsorry - UV is ultraviolet light, and moths and some other insects can see light in those wavelengths. we cannot see it without using a black light.
Bugscope TeamI did transmission electron microscopy for 5 years and then have been running labs with both TEM and SEM (what this is) for I guess about 23 years.
- Bugscope Teamthese are bacteria sitting above a biofilm that they produce to protect themselves
- Bugscope Teamthey are bacilli -- the rod-shaped bacteria
- Bugscope Teamthe other kinds of bacteria are cocci, which are round, and spirochetes, which are spiral
- Bugscope Teamspirochetes are the bad bacteria
- 10:07 am
- StudentAre the stringy things biofilm?
Bugscope Teamyes they are! they are the remnants of the biofilm after it dried.
- Bugscope Teama biofilm is normally a gel that the bacteria (not all types do this) exude and then live in
- Studentwhere did this bacteria come from? was it in the U.S?
Bugscope TeamI am sorry I don't recall, but it was from someone here who want to look at them. So yes the US.
- TeacherScott...what is a trap door on a jumping bean
- Bugscope TeamMexican jumping beans have larvae in them, and when they hatch they need to cut their way out.
- Bugscope Teamso this is the door. it is full of scales -- the moth must have really been struggling to get out
- StudentWhat's coming out of the trap door?
Bugscope Teamthose are moth scales that came off of its body as it was trying to squeeze out
- Bugscope Teamthis is a close up view of the pulvillus between the claws of a housefly
- Bugscope Teamand now, obviously, lower mag of the same thing'
- Bugscope Teamthe tiny setae, called 'tenent setae,' are sticky and responsible for the fly being able to walk on the ceiling, for example
- Studentthank you
- Studentthank u this was epic
- Studentthanks and bye
- Studentthank you very smartish
- Studentthank you this was very cool
- Bugscope TeamThank You, Everyone!
- StudentTHANK YOU TTYL
- Studentthanks man i appreciate it
- 10:12 am
- Bugscope Teamthis is fun for us
- StudentThanx scott that was totahlly awesome
- TeacherGreat job Scott - these guys are on their way out. A new group in soon...
Bugscope Teamtotally cool
- StudentIt looks like velcro!!! Thank you Scott!!!! This has been really cool!!!
Bugscope TeamThank you!
- Bugscope Teambrb
- Bugscope Teamokay we are back!
- Bugscope Teamplease let us know when you have questions
- GuestI just stumbled upon this live session what ages do you usually work with
Bugscope Teamfrom K to 12, and sometimes college
- 10:18 am
- Bugscope Teamvery young kids aren't likely to be typing questions, and they often get tired sooner, of course
- GuestOH! I am interested, I am going to collect some information this is amazing
Bugscope Teamyou're in the right place!
- GuestIs it possible to catch live sessions often? or is it better to runour own
Bugscope Teamit is possible to catch a live session twice a week. certainly it is better to run your own.
- Guestwe would collaborate
- GuestI am not the science teacher, but the tech teacher
Bugscope Teamit is often the tech teacher who gets the others involved
- Bugscope Teamwe are booked into May/June now, as a result of two kind of bursts of publicity
- GuestOkay do you have suggestions where to start? I will look at the curriculum and see where we fit,
- Bugscope Teamso you might want to apply soon and leave the dates open; Kendra can set you up
- Guestookay great, thanks!
- Guesthow long before we run a live session would we apply
Bugscope TeamI am sorry -- it is a while now. But you are always welcome to check in with us like this.
- Guesthave a great day!
Bugscope TeamThank you -- You too!
- 10:23 am
- Teacherback for round 2 - kids are just logging in now
- Bugscope Teamgood deal!
- Bugscope Teamready to roll!
- Bugscope Teamwhoa lot of students! hello!
- Bugscope TeamHi Dillon!
- Bugscope Teamthis is a fly's claw, and you can see the sticky hairs called tenent setae that enable it to walk on the ceiling, or on glass, etc. no problem
- Bugscope Teamthe pad is called a 'pulvillus,' and you can tell, pretty much, if for example a beetle is going to be able to climb, by whether it has that particular feature
- Studentthat stuff looks velcro is it?
Bugscope Teamit is sort of like Velcro the way it works, but it is composed of single sticky hairs
- Bugscope Teamnow moving up to the thorax
- Bugscope Teamand the head
- Bugscope Teamyou can see the antennae now
- 10:29 am
- Bugscope Teamthere is a pad portion with the Johnston's organ inside, and the aristate portion of the antenna, which is branched
- StudentWhy are there hairs around the eye?
Bugscope Teamthose are sensory hairs/bristles/setae much like the whiskers of a cat or rat
- StudentDo flys have eye lashes?
Bugscope Teamsort of, but their eyes do not blink
- Bugscope Teambecause they do not have skin with nerve endings at the surface, insects have setae that stick through the cuticle to help them sense their environment
- Studenti remember that these are compound eyes but why do they see better than us?
Bugscope Teamthey have much better peripheral vision -- they don't have to turn their heads as much as we do. also, all of those individual lenses -- the ommatidia -- pick up changes in the visual field very quickly, so it is hard to smack a fly, for example'
- Bugscope Teamcompound eyes are sometimes sensitive to wavelengths of light we cannot see, like UV. but of course I would prefer not to be an insect
- 10:34 am
- Bugscope Teamsetae can be mechanosensory -- touch sensitive; chemosensory, meaning that they can sense small amounts of chemicals in the air; or thermosensory, for hot/cold sensation
- TeacherWhat is this structure at the base of the antennae?
- Studentdo they see different things out of each lense
Bugscope Teamslightly different, and all of the images are stitched together in the brain
- Bugscope Teamthese are the antennae, with their two components
- Bugscope Teamyou can see the sponging mouthparts as well, and two palps that help the fly taste its potential food
- Bugscope Teamsome of the tiny setae we see are not sensory -- they serve other purposes
- Studentwhy does the fly's facets shaped like pentagons and not circles?
Bugscope Teamit's kind of like the solution to a stacking problem with round objects like oranges
- StudentWhy do the individual facets on the ant, dome shaped?
- Bugscope Teamthe best way to stack round objects is to have them form hexagons, but we see that pentagons are there as well, as in the Buckminster Fuller domes.
- Bugscope Teamit's about the size of a large desk, like an executive desk, with a 6-foot column on one end.
- Studenthow large is this microscope?:)
Bugscope Teamit has its own chilled water, filtered electrical service, nitrogen and air...
- 10:40 am
- Teachernice bucky ball reference!
Bugscope Teamha yeah the precursor to nanotubes and now graphene
- Bugscope Teamthis is one of the spiracles on the thorax of the stinkbug
- StudentHOW FAR CAN YOUR MICROSCOPE ZZOOM IN?
Bugscope Teamthis one can go to a million x but for a publishable image in a research paper the max is about 250,000x.
- Studentwhy do they have a different breathing system?
Bugscope Teamthat is the way they started out, and it has not changed, fortunately for us. if insects had better respiratory systems they would be larger and more dangerous to us
- Bugscope Teamthey have what we consider a 'primitive' breathing system that limits their size; when the earth was younger and there was much more oxygen in the air, the insects could be larger as well
- Student nm=/
- 10:45 am
- Studentwhy does the microscope not show you in color?
Bugscope Teamwhat we are seeing is 'signal,' in gray scale. it results from us beaming electrons at the sample that knock what are called secondary electrons out of the surface of the sample. we collect the signal from the secondary electrons in shades of gray.
- StudentDoesnt their chiten exsoskelton limit their size?:):)
Bugscope Teamin a way it does. some arthropods will undergo numerous molts as they become larger. once an insect reaches a stage at which it has wings, it is done
- StudentWhy can we zoom in farther with a electron microscope?
Bugscope Teamwe can see better because we are using an electron beam that is much smaller than the wavelengths of light. it is like the difference between playing a piano with mittens on compared to using your fingers -- obviously you have more sensitivity and can touch more keys with your fingers
- Studentthis looks like a sucking mouth what does it eat?
Bugscope Teamit pierces the leaves and stems of plants and sucks out plant juices
- Studentwhy do they call a stink bug a stink bug?
Bugscope Teamit produces chemicals that smell bad and discourage other insects and birds, etc. from biting it or getting any closer
- 10:50 am
- Bugscope Teamthese are bacilli, which are one of the three basic shapes of bacteria
- Studentwhat are those strings around the bacteria?
Bugscope Teamthe very fine strings are the remnants of a film the bacteria had produced that protected them; the larger strings I am sorry -- I am not sure what they are from.
- Bugscope Teamsome bacteria produce a biofilm that is like a gel they can swim around in. it protects them from drying out and also from getting washed out of your vegetables, for example
- Bugscope Teamsome of the bad bacteria that are responsible for diseases live in biofilms that are hard to wash away
- Bugscope Teamthere are, generally: bacilli -- the rod-shaped bacteria; cocci -- the round ones; and spirochetes, which are spiral
- Bugscope Teamspirochetes are often the very bad bacteria
- Bugscope Teamfound in diseases like syphilis and also gum disease
- StudentIs this bacteria in the process of duplicating?
Bugscope Teamyes I am sorry this is not well preserved, but yes
- 10:56 am
- StudentWhy dont bacteria get larger:)?
Bugscope Teamthey reach a useful limit in size and then reproduce themselves; there must be some advantage in remaining small like that
- Bugscope Teamwe can get much better images of bacteria, and I am sorry the ones I put in the 'scope today were not nearly as good as some we have seen
- Bugscope Teampart of the deal is that I did not want to take someone's bacteria without permission
- StudentWhat are the stingers of hornets and bumble bees made of?:):):)
Bugscope Teamthey are made of chitin but may have, I believe (like the mandibles of some insects) metals in them that help harden them
- Bugscope Teamchitin is like what our fingernails are made out of, or you could compare it to shrimp shells, which are the same thing
- 11:01 am
- Bugscope Teamthis is a Mexican jumping bean, which is where a certain kind of moth larvae live until they become moths
- Studentwhy do they call it the mexican jumping bean?
Bugscope Teambecause it has a larva in it that is alive, if you heat it a bit, it will jump around
- Bugscope Teamthe moth is to the right, or one of the moths
- Bugscope Teamthese are salt crystals from a Wendy's restuarant. they have a cool incised shape unlike normal salt crystals
- StudentThank you:):):):):)
- Studentthank you a million SJ!!!!!!!!!!!!!!!!!!!!!!!!!!!!
- Studentthank you that was really cool you guys are awesome
- StudentThis was so awesome thank you!
- StudentThank you for the presentation it was really cool. I want to do it again. Thanks SJ.
- StudentThank you! You are very knowledgeable
- Bugscope TeamThank You!
- StudentThanks, the show was awesome!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! :D
- Bugscope TeamThis is really fun for us.
- StudentThanks for being fantastic:):):):)
- StudentThank you!!!!!:)
- Bugscope TeamYou were driving a $600,000 microscope.
- TeacherSJ - that is the end of this class - fantastic!
Bugscope Teamis that it for today?
- Bugscope Teamdo you have more classes coming in later?
- Bugscope TeamThank You, Everyone!
- TeacherThis next period is an elective for our sevent graders. I have another class that comes in at the end of the hour - but I think we run out of time for our session, correct?
- 11:07 am
- Bugscope TeamMr McG we had planned to run for another hour, 'til 12 our time. Does that work for you?
- Bugscope Teamactually I can go 'til 1 my time if I get some food...
- Bugscope Teamso just short of 2 hours more
- Bugscope Teamup to you...
- TeacherSJ - well we don't have another class until 11:58 so if you can go to 1pm your time that would be great and we can run one more class!!! See you in about 45 minutes.
Bugscope Teamlet's do it
- Bugscope TeamI'm heading up for food and plan to be ready in 45 as you said.
- 11:24 am
- 11:49 am
- 11:55 am
- Bugscope Team(driving around looking for more presets...)
- 12:02 pm
- TeacherOK...back with the next group. a few minutes as the get logged in!
- Bugscope TeamCool!
- Bugscope TeamHello K Roche and Jogger!
- Bugscope Teamwe are ready to roll
- Bugscope TeamJerry is likely gone...
- Bugscope Teamplease let us know when you have questions
- 12:07 pm
- Bugscope Teamthis is a very small ant -- you can tell in a way by the scalebar
- Bugscope Teamits head (her head) is only about a half millimeter in diameter
- Studentwhy does this microscope take bettter images than a standard microscope?
Bugscope Teamnormal microscopes use light and glass lenses; we are using electrons, which are very small, and electromagnetic lenses to focus the electron beam
- Bugscope Teamnormal light microscopes are limited by the wavelengths of light
- StudentDoes a Ants eye have a lens
Bugscope Teamit has a number of lenses, which is what the ommatidia -- the facets we see now -- are
- Bugscope Teamthe ant gets a kind of mosaic image of what is around it
- Bugscope Teamants depend more on their antennae to get information about their environment than their eyes
- 12:12 pm
- Bugscope Teamif you had compound eyes you wouldn't have to turn your head as much to see what is around you
- Studentwhy are these in black in white and not color?
- Studentare those dark spots holes? and what part of the wing is this
Bugscope Teamthis is one of the scales on a wing, and the dark spots are holes in between the lattices
- Bugscope Teamthis is a section of a Monarch butterfly wing
- Bugscope Teamthe scales are what make the wings feel so silky to us; they are what we see as the 'powder' that falls off of the wings when we rub them
- Bugscope Teamwing scales have a number of purposes, and one is to produce the colors we see
- Bugscope Teamthe spacing between those ridges produces structural colors
- 12:18 pm
- StudentWhat happens after the electrons hit the object?
Bugscope Teamthe high energy electrons that hit the object cause what are then called secondary electrons to be ejected from the object. the scanning electron microscope collects the signal from the secondary electrons
- Studentis there like a certain pattern?
Bugscope Teamin the scales? yes, definitely, and it is responsible for different colors
- Bugscope Teamto the top of this view is a dome like object that is actually an ocellus -- a simple eye
- Bugscope Teamflying insects have, usually, three of them on the top of their head
- Bugscope Teamthey are simple eyes, not nearly as good as compound eyes; they see light and dark but help the insect maintain its orientation with respect to the sun as it flies
- Studentis that the eye
Bugscope Teamyes that little dome was one of the ocelli -- the simple eyes
- Studentwhat is the purpose of the hairs
Bugscope Teamthe hairs, which are often called 'setae,' pronounced see-tee, are sensory; their purpose is to convey information through the cuticle to the nerves beneath
- Bugscope Teamsetae can be mechanosensory, like cat or rat whiskers; they can be thermosensory, registering hot/cold; and they can be chemosensory, used to sense smells in the air, such as pheromones, which are like perfume, in a way
- 12:23 pm
- Studentgot to go
- Studentwe gotta go thanks
- Studentwe got to go thanks it was great
- Studentdo all compound eyes have the same pattern?
Bugscope Teamno. some are more complex and have different capacities for collecting light
- Studentsorry we have to go thank you :)
- StudentThank you this was a lot of fun!
- Studentthank you
- Studentthank you very much but we have to go
Bugscope Teamoh no!
- Studentwe all have to go thank you vary much bye
- Studentwe have to go thank you christa and becky :)
- Bugscope TeamThank You!
- Studentbye! thanks alot! that was amazing:) byeeeeee:)
- Studentthanks a million
- StudentThank you for showing us the electron microscope pictures.
- Studentthank you this was a really cool experience and fyi my mom is a scientist and she will probably think this ais craxy cool
- Studentthanks again
- Bugscope TeamThank you for logging in today!
- Studentthank man you rock pice
- TeacherScott - oops - another teacher in to use the lab so we are getting the boot. I thougt it was open. Thanks for offering to extend the session for these guys - very cool for them to see. Thanks again for another great session!!!
- Bugscope Teamhttp://bugscope.beckman.illinois.edu/members/2011-114
- Bugscope Teambelow is your member page, in which you can see my typos and questions I missed, plus the good stuff
- Bugscope TeamThank You!
- Bugscope Teamokay unless one of our guests would like to drive, I am shutting down for today.
- Bugscope Teamover and out...
- Bugscope TeamThank you, everyone!
- Bugscope TeamBye!
- Teacherstill there? just jumped to my room and we will project. let me know