auburndale hs
Session 2008-010

Application

School

Class

Project

Miscellaneous

Transcript

Connected on 2008-03-14 08:00:00 from Auburndale, WI, US

• 6:56 am
• Bugscope Teamrxl started, session enabled
• 7:38 am
• Bugscope Teamvac started
• Bugscope Team1.4...
• 7:44 am
• Bugscope Teamvac good, starting presets
• Bugscope Teamyay!
• Bugscope Teameyeball

• 7:49 am

• Bugscope Teammothman

• 7:55 am
• Bugscope Teamhi mark, welcome to bugscope!
• Bugscope Teamwe are still finishing up some presets, done real soon
• TeacherHello what are we looking at today
• Bugscope Teamwell, we've got several insects for your viewing pleasure. a moth, grasshopper, shield bug, lady bug and a beetle
• Bugscope Teamwe are goign to make one more preset and then i'll unlock the session
• Bugscope Teamanti
• TeacherOk I am just looking at my controls!
• Bugscope Teamcool, they won't work until we unlock the session

• Bugscope Teamdoes everything make sense so far?
• Bugscope Teamwe're giving you control...
• Bugscope Teamokay, presets are done, session unlocked. mark, you have control now
• Bugscope Teamthe current image is an ant head
• Bugscope Teamyou can at any time click on a preset to take you to another insect
• Bugscope Teamor change mag, drive around where you are
• Bugscope Teamif you have any questions please feel free to ask us
• Bugscope Teamif you click to drive you need also to click to stop
• 8:02 am

• Bugscope TeamHI all

• Bugscope Teamhey annie

• Bugscope TeamYay Annie@

• Bugscope Teamthis ant has ocelli
• Bugscope Teamthat is a paper tab in the background
• Bugscope TeamAnnie with a trademark.

• Bugscope Teaminsects have compound eyes, which are very interesting, they are composed of hundreds of individual facets, called ommatidia

• Bugscope Teamha ha ha

• Bugscope Team40x is close to the minimum resolution, i think
• Bugscope Teamthose three bumps are the ocelli
• Bugscope Teamyes, notice the small bumps to the left

• Bugscope Teamthe bumps are ocelli, they are also called simple eyes

• Bugscope Teamyou can see where the left antenna is busted off
• Bugscope Teamants generally get much more information from their antennae than they do from their eyes
• Bugscope Teaminsects are also very hairy. those hairs are called setae (pronounced sea-tea). setae can be mechanosensory or chemosensory. setae help the insect to sense it's environment
• TeacherHow much does a SEM cost?
• 8:07 am
• Bugscope Teamthis one was around $600,000
• Bugscope Teama little over 9 years ago
• Bugscope Teamyes, and you are controlling it live!
• Bugscope Teambut don't worry, it is not possible to damage it

• Bugscope Teamsometimes the scope gets confused, but we can kick it back into gear ;)
• Bugscope Teamthis is a particularly nice SEM because it has a field-emission electron gun (an extra $200,000 item) as well as wet mode capability
• Bugscope Teamwe can image uncoated nonconductive samples using this 'scope

• Bugscope Teamnice driving mark

• Bugscope Teamand we can work at 100% relative humidity

• Bugscope Teamthough it is a lot more difficult to work in wet mode which is what we call it when 100% humidity
• Bugscope Teamthis microscope has 2 nm resolution -- with a perfect sample and under perfect conditions
• Bugscope Teameach eye facet has a lens and can see a strait line outwards. the curvature of the entire compound eye ensures that the insect can see a wider range than just a strait line in front
• Teacheris magnification multiplied

• Bugscope TeamUm we can change the mag however we want
• Teachercan we go to a different specimen
  Bugscope Teamat any time you want, you can click on a preset, and it will move to that location

• Bugscope Teamtry another preset

• 8:12 am

• Teacherwhat is that

• Bugscope Teamyou can also see a couple of pollen grains in there

• Bugscope Teama lot of these samples came from an entomologist's collection, so you will see a pinhole through the thorax
• Teacherwhere are the pollen grains
• Bugscope Teamor as we saw with the ant, a paper tab
• Bugscope Teamthey were the little balls on the claw. so if you took the mag back up you will see them again
• Teachernew driver: this is echko
• Bugscope TeamHi Echko!
• Teacherhow many times can this SEM magnify?
• Bugscope Teamwe can go over 800,000x
• Bugscope Teamit can pretty high but you wont see anything interesting beyond 200,000x most of the time

• Bugscope Teaminsects especially we cant go very high with

• Bugscope Teambut for meaningful, publishable images we rarely go over 200,000x, as Cate says

• Bugscope Teamwith insects it's easier to get good images anywhere from 40x to 30,000x
• Bugscope Teamthey dont have very interesting features at high mags

• 8:17 am
• Bugscope Teamwhen we use the microscope for bugscope we have the sample at a long working distance so you can image more of the insect at low mag
• Bugscope Teamand with a long working distance we don't have quite the resolution
• Bugscope Teamthis is the hole where the pin had gone through, of course

• Bugscope Teamthis is a shield bug which is a true bug

• Teacherwhat are the four of you majoring in?
• Bugscope Teamhere you can see where one of the antennae is missing
• Bugscope TeamI am working on my PhD in entomology
• Bugscope TeamI got a degree in English and Biology. A long time ago.
• Bugscope Teamscott is the senior electron microscopist here, he's past schooling
• Bugscope Teami majored in physics
• Bugscope Teami have two degrees in music
• Bugscope Teambut i'm a sysadmin now
• Bugscope Teamannie is the real entomologist here though
• Bugscope TeamAlex is our sysadmin, and an uncommon one you can actually talk to and who actually likes to work
• Teacherwe are a freshmen-sophmore biology class
• Bugscope Teamvery cool, your teacher is excellent for trying to find interesting projects for you all
• Bugscope TeamI became interested in entomology when I was in high school
• Bugscope Teami liked bio in high school a lot
• Bugscope TeamUntil my senion year I wanted to be an opera singer
• Bugscope TeamOr a musical theater actress
• Bugscope TeamI took biology because I was in danger of becoming an english teacher
• TeacherThis is Reba
• Bugscope TeamMy parents would have killed me!

• Bugscope TeamHi Reba!
• Bugscope Teamhi reba! welcome to bugscope
• Bugscope Teamhey reba!
• 8:22 am
• Bugscope Teamclick on another preset if you would like

• Bugscope TeamReba, let us know if you have any questions about what you see on your screen

• Bugscope Teamyou can drive around using the navigation buttons or you can go to another insect by clicking on the preset picture
• TeacherOur computer is slow, bear with us

• Bugscope Teamthe insects were sputter coated in gold so you wouldn't see much color anyway

• Bugscope Teamthe images are all black and white because this is an electron microscope: the image is gathered via electrons that are bouncing off the insect, within a vacuum, and are collected by a secondary electron camera

• 8:27 am
• Bugscope Teamhere you can see some of the tenent setae
• TeacherAre there are interesting projects you are working on with the SEM?
• Bugscope Teamwe train people to use the microscopes to do their own research

• Bugscope Teamwe train grad students, mostly, and postdocs, and sometimes undergraduates
• Teacherwhat do the tenet setae do?
  Bugscope Teamthe tenet setae help the beetle to adhere to surfaces

• Bugscope Teampollen
• Bugscope Teamand the tenent setae
• Bugscope Teamthere you can see the setae better and the pollen ball

• Bugscope TeamI use the SEM to look for pores that are associated with pheromone release in longhorned beetles. I think it is interesting.
• Bugscope Teamthe setae we see are the ones that allow the beetle to stick to the ceiling, or to glass, when it walks

• Bugscope Teamif you are having problems with click to drive, sometimes click to center will give you better control

• Bugscope Teamyou can pretty much tell from looking at the tarsi whether a particular insect will be able to walk up a wall, for example
• Bugscope Teamthe other setae we see are mechanoreceptors -- like cat whiskers -- and chemoreceptors
• 8:33 am
• Bugscope TeamThese partticular setate look like mechanoreceptors to me
• Bugscope Teamoops, particular

• Bugscope Teamyeah they are fluted, so they are sort of (designed!) to be able to bend in any direction

• Bugscope Teamthe hairs are the "setae" that we are talking about. setae is plural, seta is singular
• Bugscope Teamso yes as Annie says these are very likely mechanoreceptors

• Bugscope TeamI would say that mechanosensors are the most common setae on most surfaces

• Bugscope Teamtheir exoskeleton is like a suit of armor and in order for them to feel whats going on is to have these setae stick through the armor. the setae are connected to nerves

• TeacherDo you do any work with honeybee mites?

• Bugscope Teamwe have some of them in the hood here but they are really gummed up.

• Bugscope Teambut we have not had any projects involving them
• Bugscope TeamHoney bee mites are kind of like tomatoes: any of them you can get in the winter are not worth having
• 8:38 am
• Bugscope Team(I say that, but I buy tomatoes in the winter)
• TeacherWhat are the spear shaped things on its eye?
• Bugscope Teamthose are scales

• Bugscope Teamanalogous to feathers in birds

• Bugscope Teamscales can be responsible for color patterns; they can help provide lift; and they can help the moth get out of webs

• Bugscope Teamthey're what make butterfly wings feel silky

• Bugscope Teamthe tongue of the moth is all rolled up

• Bugscope Teamall of the chat and images collected during this session are saved onto your bugscope member webpage: http://bugscope.beckman.uiuc.edu/members/2008-010

• Bugscope Teamthe arrangement/spacing of the tiny ribs in the scales can produce structural colors -- colors that are not related to pigments

• TeacherHow long is the tounge compared to the body?

• Bugscope Teamwell, good question, trying zooming out a bit and maybe we can get an idea

• 8:43 am

• Bugscope Teamalso notice the measurement scale in the bottom left of the image
• Bugscope TeamI think it depends, of course, but in some cases the tongue can be quite long
• Bugscope Teamcompared to the length of the body
• Bugscope Teammm is millimeters of course. um stands for microns. 1 um = one millionth of a meter.
• Bugscope Teamit looks like if the proboscus was unwrapped, it'd be about 3-4 mm long
• Teacherwhat is the broken structure beneath the eye?
• Bugscope Teamthat is the base of one of those palps
• Bugscope Teamyou can see an unbroken one to the left

• TeacherThe grasshopper doesn't work.
• Bugscope Teamlet me try

• 8:48 am

• Bugscope Teamyep, scope is confused, just a sec please
• Bugscope Teamscope is confused, fixing now
• Bugscope Teamcate fixed it, thanks cate!

• TeacherThis is mark
• Bugscope Teamhi mark
• Teacherclass has ended could you take a couple screeen shots of the grasshopper for discussion on monday?
• Bugscope Teamgrasshoppers don't work as a rule ; )
• Bugscope Teamyes mark, all images are saved automatically to your member page: http://bugscope.beckman.uiuc.edu/members/2008-010
• Bugscope TeamMark if you have time you can drive around it. Or we can just cruise a little to add images to your session.
• Bugscope Teaman image is saved whenever the image is still for 2-3 seconds
• Bugscope Teamalso, all the chat is there as well

• Bugscope TeamThanks Annie!
• Bugscope Teamthanks mark!

• 8:53 am

• 8:59 am

• Bugscope Teamsmushed piece of dirt or juju
• 9:10 am
• Bugscope Teamalright, good session everyone, stopping rxl, disabling session now