Connected on 2007-11-13 12:30:00
from , VA, US
- 12:29 pm
- Bugscope Teamare you in Washington, Abe?
- Guestno
- Guestwow!!these pictures are unbelievable
- Guesti'm from florida
- Bugscope TeamCool!
- Bugscope Teamcate is repsonsible for the cool images today :)
- Guestwow!!cool!! great
- Guestgood job Cate
- Bugscope TeamHello Crystal!
- Bugscope Teamthanks!
- Bugscope Teamwelcome to bugscope crystal
- Bugscope TeamGreetings to NSF CDC. Hello Sara!
- Guestwhat will happen to the bug when you pass electron beam??
Bugscope Teamthe bug is almost always already dead by the time it's in the scope. we coat the bugs with gold/nickle palatium, and that would probably kill them.
- Bugscope Teamthe electron beam is giving us the images we see
- Bugscope Teamso we hope it doesn't do anything adverse
- Guestno i mean is it possible to keep the insect/ bug./cell alive??
- Guestoh!!
- Bugscope Teamthe bug could be alive -- it could hold its breath by closing its spiracles
- Bugscope Teamit is, in wet mode, and sometimes in hi-vac as long as the bug can hold it's breath, but it's hard to image them when they move around
- Bugscope Teambut it would eventually die
- Bugscope Teamthere have been cases where some bugs are alive inside the vacuum scope, right scott?
- Bugscope Teamand of course as Cate says it could be moving around, and then we would not be able to image it
- Guestits amazing
- 12:35 pm
- Bugscope Teamyes we have had, for example, a Monarch butterfly chrysalis in the 'scope for 4 hours, and later on it hatched
- Bugscope Teamafter we had taken it out of the 'scope
- Bugscope Teamit was coated with gold-palladium too
- Guesti hav a term project based on the wet technique
- Guestwow!! tats a true survival story!!
- Bugscope Teamwe run the 'scope in HiVac mode for this of course
- Bugscope Teamwet mode is pretty slow, often better to average to get good images
- GuestHow will you coat gold or palladium??
- Bugscope Teamor run the beam more slowly than usual
- Bugscope Teamwe have a sputter coater with a turbo vacuum pump, and we sputter the alloy gold-palladium onto the samples
- GuestHi! My school is doing this on Thursday, so I thought I'd check it out. This is incredible!
Bugscope Teamvery cool! we are looking forward to your session on thursday crystal.
- Bugscope TeamI don't remember what the percentage is of gold and palladium
- Bugscope TeamCrystal thank you for chiming in. We look forward to seeing you.
- Bugscope Teamthe logos prep academy right?
- Bugscope TeamNo one is driving right now.
- Bugscope Teamso I just changed to a new preset
- Bugscope Teamwhen you are on with us you are able to drive the microscope.
- Bugscope Teamack! sorry, i just gave control to abe. abe you should now see controls for the scope on the right side of your browser window
- Bugscope Teamthat means you can have a very dynamic session, driving all of the time
- Guestoh!! ok..
- Guest how to use tat
- Bugscope Teamchange mag, move, adjust contrast/brightness, focus
- Bugscope Teamyou can even run off the stage and make the same mistakes we do
- Bugscope Teamnormally you would login as the teacher, and the teacher is automatically given control of the scope.
- 12:40 pm
- Bugscope Teamso now abe is driving
- Guestwow!! cool..thanx
- Bugscope Teamwe can confer control to anyone who wants it
- Bugscope Teamone person at a time
- Bugscope Teamthis is a pseudoscorpion that one of the faculty here in the building brought us
- Bugscope Teamso it is not an insect...
- Bugscope Teamthis is the first time we have seen one of these
- Guestwhat is it then???
- Bugscope Teamnormally by now the people we are working with would have logged in -- we don't know what has happened to them
- Bugscope TeamHi everyone!
- Bugscope Teamum Good Annie is here -- she knows everything.
- Bugscope TeamEven about noninsects.
- Bugscope TeamAnnie is our entomologist.
- Bugscope Teamwe have to keep Annie occupied or she will multitask during the session.
- Bugscope Teamha ha ha
- Guestoh!!hi Annie..
- 12:45 pm
- Bugscope TeamAnnie is super high energy and cannot sit still for long.
- Bugscope Teamwhen we make a preset like this we are tempting you to take the mag lower to see where you are.
- Bugscope TeamHi Abe
- Guestyeah
- Guestthis is really cool
- Guestthere are two insects in this image
- Bugscope Teamyup, we have two little flies
- Bugscope Teamhere is a close up of a fly's compound eye
- Bugscope Teamand this is tarsal claw...insect toes!
- Guestcan you share some of your interesting experiences while taking these images?
- Bugscope Teamokay, going to switch control of the scope to bluewhales now
- Bugscope Teamyes, do you have any specific questions?
- Bugscope Teambluewhales, welcome to bugscope!
- Teachersorry for joining so late, we are from nsf cdc
- Bugscope Teamhello bluewhales
- 12:50 pm
- Bugscope Teamnot a problem, i just tried calling you but couldn't get to your phone...
- Guestthanx for letting me control the drive
Bugscope Teamno problem abe!
- Teacherwhat are we looking at
- Bugscope Teamthis is a claw on a beetle, magnified 205 x
- GuestHi!!blue whales
- Bugscope Teamannie might call this a tarsi, that just means it is an insect foot
- Bugscope Teamannie is our local entymologist
- GuestAbout the specimen preparation , mode used, resolution, maximum magnification
- Bugscope Teamentomologist!
- Bugscope Teamyeah i know...i suck at spelling
- Bugscope Team;)
- Bugscope Teami am watching you alex ;)
- Bugscope Teamuh oh
- Bugscope TeamInsects!!
- Teacheri have a question?
- Bugscope Teamask away!!
- Bugscope Teamgo ahead bluewhales
- GuestAbout the specimen preparation , mode used, resolution, maximum magnification
Bugscope TeamThe specimens are placed on double-sided carbon tape, grounded with silver paint, and coated with gold-paladium. We use standard high-vac mode (not wet mode). Scott, Cate..can you answer about the resolution and max. mag?
- Teacherhas anyone ever used this program with preschoolers?
- Bugscope Teamwe usually have the teacher run the controls with preschoolers
- Bugscope Teami'm not sure bluewwhales, let's see what scott says
- Teacheri could imagine. thanks
- Bugscope Teamthere is a variety of ways the teacher can decide to run the session
- Teachercan we send in our own insects?
Bugscope Teamyes of course! we love to see the different kinds of insects schools send
- 12:55 pm
- Teachercan the still be alive?
Bugscope TeamThey will usually not be alive when we get them AND sometimes they will eat each other.
- Bugscope Teamwith preschoolers often the teacher runs the 'scope, and lately teachers have been using smartboards
- Bugscope Teamplease do not send live insects
- Bugscope TeamGreetings to NSF CDC: Hello to Sara
- Bugscope Teambut we are happy to have you send insects/bugs/arthropods
- Teacherthank
- Bugscope Teamif they are alive, you can freeze them, and they just go to sleep
- Bugscope Teamit is best to choose small critters -- rather than grasshoppers or cicadas or giant moths
- Teacherhow long is the turn around between sending the insect and the actual live session
- Bugscope Teamyou decide when the session will be
- Teacherok
- Bugscope TeamKendra does the scheduling with you via email
- Bugscope TeamBluewhales: Please give myrregards to Sara.
- Bugscope Teamso if the samples are dry we could get them the day before the session
- Teacherthis is our first time on the live session
- Teacherwe will give your regards umesh
- Bugscope Teamif they are not dry the 'scope may not pump down
- Teacherwhat are we viewing at this present moment
- Bugscope TeamBluewhales: There was a live session/demo on Oct. 2 via NSF Library with Sara. It will be great if the students help in bug collection as well.
- Bugscope Teamplease ask us questions about anything -- we have been doing this for 8 1/2 years.
- Bugscope Teamand we have gone through changes in the setup, the software, hundreds of schools
- Bugscope Teamon your end you have a lot of options regarding how you want to run things
- 1:00 pm
- Bugscope TeamI'm not sure what we are seeing right now, you could try to lower the mag a little bit so we can tell what this is
- Bugscope TeamBluewhales: We have had K to 12 (and college) students use Bugscope. I think with the new interface it will be great to have NSF CDC involvement. We also have a CDC here at Illinois. So, it may be good in future to have students from here and students from Arlington work on this.
- Bugscope Teamthis is a bug we did not recognize -- some sort of beetle
- Guesthi!!! can anyone answer about the max resolution and magnification for this high vaccuum mode?
- Bugscope Teamif you drive to the head I might because able to identify it
- Bugscope Teamyou can see now that we were looking at the tarsi -- the forearm joints
- Bugscope Team2 nm
- Bugscope Teamfor magnification, this scope can go to about 200,000x to see anything useful
- Bugscope Teamyou can get meaningful images at 200,000x although you can go much higher
- Bugscope Teamhmmm
- Bugscope Teamthis is a field emission 'scope -- it has a field-emission electron gun
- Bugscope TeamAnnie is trying to figure out what this is
- Bugscope Teamso yeah the ultimate resolution is 2 nm
- Bugscope TeamMy best guess is a soldier beetle...a cantharid
- Bugscope Teamall of the images you obtain are saved to the database for your session
- Bugscope Teamthat is what cantharides come from?
- Bugscope Teamcantharidin comes from cantharidae
- Bugscope TeamSpanish fly.
- Bugscope Teamyup
- Bugscope Teamwell no one is going to eat this
- Bugscope TeamI am not 100% sure it is a cantharid though
- Bugscope Teamwe could dare Alex to...
- 1:05 pm
- Bugscope Teamum, what?
- Bugscope Teambluewhales please let us know whenever youn have a question or a problme
- Bugscope Teami just ate a big cheeseburger, i'm sure there were some dead bugs in there....
- Bugscope Team"canthar" is a prefix that sometimes refers to beetles in general...such as cantharophilous flowers: those that are pollenated by beetles
- Guesthey!!its very nice to see u all guys.. when is the next session?
Bugscope Teamabe, you can check for session on the top of the bugscope website: http://bugscope.beckman.uiuc.edu
- Bugscope TeamCrystal's session is 1 to 2 our time on Thursday.
- Guestoh!!thank you..now i'm leaving ..bbye all
- Teacherthanks so much for the walk through. We appreciate your timely responses. We are signing off. Have a great day. TTY SOON.
- Bugscope TeamSo we will be on before that, setting up.
- Bugscope Teambye bye bluewhales, great to have you on!
- Bugscope TeamThank You!
- Bugscope TeamCrystal: I hope you are ready and excited about your session.
- Bugscope Teamcrystal is gone umesh, you can tell that by the greyed-out name
- Bugscope TeamHello Annie: I hope the Noyce presentation slides were helpful. I am working on a plan for another presentation. I would like to talk with you when convenient.
- Bugscope TeamOk Umesh. Let's discuss a time over email
- Bugscope TeamI am free Thursday all day and Friday afternoon
- Bugscope TeamI think the person who logged in from CDC is working for Sara (who directs CDC). So, this may have been a test session.
- 1:11 pm
- Bugscope TeamAnnie: Is there a time on Thurs. that works best for you? Can we meet at NCSA?
- Bugscope Teamwell, we have a session at 1 pm, right?
- Bugscope Teamtoday?
- Bugscope TeamThursday
- Bugscope Teamyes yes, thursday at 1PM
- Bugscope Teamhttp://bugscope.beckman.uiuc.edu/admin/test_results.php
- Bugscope TeamBen: Check your door
- Bugscope Teamare we ready to close the session and get back to work?
- Bugscope Teamyeah let's give Cate the 'scope
- Bugscope TeamAnnie: Well, let us meet closer to where you are? How about the Union?
- Bugscope TeamOK...the union will work
- 1:16 pm
- Bugscope TeamBen: The frame was the only one I had, but the card is new from Phillips (see note on the back).
- Bugscope TeamAnnie: Thanks I will see you at 12noon at the Union on Thurs.
- Bugscope TeamThanks Girl Pirate.
- Bugscope TeamScott: I am not following here.
- Bugscope TeamUmesh it is all good. What happened to the session today?
- Bugscope TeamThey did ask whether we had ever worked with preschoolers.
- Bugscope TeamI think the CDC wanted a test and they got one. I think it is important to them as the children are very young (ages 4 and up). Most of the children there are of program officers. So, everything is carefully done there.
- Bugscope Teamclose session? get back to work?'
- Bugscope TeamBye
- Bugscope Teamand we told them the teacher usually drives for the students
- Bugscope Teami'll go upstairs
- 1:22 pm
- Bugscope Teami'm back, logged in from my office. just let me know when you all are done and i'[ll close the session
- 1:29 pm
- Bugscope Teamthanks Alex.
- Bugscope TeamAlex let's just stop now, unless someone else wants to work with this. Cate is taking high-res images.
- Bugscope TeamI am logging out
- Bugscope Teamhi crystal.
- Bugscope Teamwait is Crystal back?
- Bugscope Teamwe were just about to close the session. did you want to drive more?
- Bugscope Teamyay!
- Bugscope Teamcrystal has control of the scope.
- Bugscope TeamI don't think Crystal ever got to drive.
- Bugscope Teamoh wait, cate is driving the scope from the esem
- Bugscope TeamCate is stopping.
- Bugscope Teamscott, can you get cates attention. i've locked the session, we can't let crystal drive until cate is not running the esem
- Bugscope Teamexactly
- Bugscope TeamAlex Cate is not driving now.
- Bugscope Teamyeah I stopped and put it back as best i could
- Bugscope TeamShe knows Crystal is on.
- Bugscope Teamokay, session is unlocked
- Bugscope Teamcrystal you have control!
- Bugscope TeamCool.
- GuestI would LOVE to drive it if you still have time! I am so worried I won't know what to do on Thursday! My kids were just finishing up a test!
- Bugscope TeamYou have control and are free to drive.
- Bugscope Teamtake some time now, i can hang out with ya
- Bugscope TeamPlease let us know when you have questions.
- Bugscope Teamthe presets, because we were dinking with some things in the meantime, may not go to exactly the right place anymore.
- 1:34 pm
- Bugscope Teamthis is one of the claws of the pseudoscorpion, which is an arachnid
- Bugscope Teamthe rest of its body is below
- Bugscope Teamto the south
- Bugscope Teamwhen you click to drive, and then click to start moving in a particular direction, you need to click the mouse again to stop driving
- Bugscope Teamlet us know if you are having trouble negotiating the sample
- Bugscope Teamgood job centering on the mouth
- Bugscope Teamnow you can take the mag up if you wish
- GuestThank you - we weren't sure we were looking at a mouth
- Bugscope Teamyou know we have never seen one of these before either
- Bugscope Teamcool
- GuestWhat kind of insect is this?
- 1:39 pm
- Bugscope Teamit is related to spiders -- it is an arachnid
- Bugscope Teamthis is a pseudoscorpion or fake scorpion, as scott said, it is a spider
- Bugscope Teamit is a very different looking spider that is for sure
- Bugscope Teamit does not have a stinging tail like a scorpion
- Bugscope Teamnice
- Bugscope Teamthe hairs are sensory setae
- Bugscope Teamthey help the little dude feel its food
- Bugscope Teamnow we are looking right into the mouth
- GuestThis is cool! We just read about setae!
Bugscope Teamthat is great! annie, our local entomology will be so happy to hear that when she does the bugscope session with your school
- Bugscope Teamyou can adjust the focus if you want
- Bugscope Teamif you make a change in one direction and it gets worse, do like you are doing and go the other way
- Bugscope Team*entomologist
- Bugscope Teamwhen I try to tell you what to do you have already done it
- Bugscope Teamhere you can see the little "hands" on either side of the opening, that is so wild
- GuestAre we looking inside his mouth?
- Bugscope Teamyeah it is just bizarre
- GuestAre those palps?
- Bugscope Teamright into its mouth
- Bugscope Teamyes they are
- Bugscope Teamsome palps have chemosensors on them
- Bugscope Teamso the insect/arthropod can taste its food
- 1:44 pm
- GuestThank you - weren't sure what chemosensors were. I now have 10 kids around me, and we're all drooling!
- Bugscope Teamthey're like tastebuds, sort of, to us
- Bugscope Teamoften a seta will have a single tiny pore at its tip, and that is a chemosensor
- Bugscope Teamthis is all black and white because we are using electrons rather than light to collect the images
- Bugscope Teamand you are driving a $600,000 electron microscope from your school
- Bugscope Teamnormally if you get lost you can go to a preset to return to 'civilization.'
- Bugscope Teamhere we have messed up the original presets, but we will not do that Thursday.
- Bugscope Teamthe electrons that are used to gather these images are smaller than the wavelength of light that human eyes can see.
- Bugscope TeamWe are so glad you came back!
- Bugscope Teamyes so as Alex says we do not see color because color comes from the wavelengths of visible light
- Bugscope Teamnice!
- Bugscope Teamyou can see it does not have a stinging tail like a real scorpion
- 1:49 pm
- Bugscope Teama tiny hand
- GuestWe were thinking the same thing. Does it have spinnerets? Were those by its mouth? Also, were we looking at the eye earlier?
- Bugscope TeamI imagine its spinnerets are at the base of the abdomen
- Bugscope Teamwe mounted the little dude right on the base of its abdomen and thus are not able to see what is there
- Bugscope Teamyou are good at this
- Bugscope Teamthere is a preset of spider spinneretes, preset #4
- GuestYou are so kind!
- Bugscope Teamyeah, crystal, you are gonna do great on thursday
- Bugscope Teamnormally this would not happen but we goofed up the presets
- Bugscope Teamthis is silver paint, so it is near the sample
- Bugscope Teamif you take the mag down further oytu may be able to see where you are
- Bugscope Teamthis is the edge of the world
- Bugscope Teamthrow a coke bottle over the edge...
- 1:54 pm
- Bugscope Teamnow we are looking for samples...
- Bugscope Teamthis is a barren place, isn't it?
- GuestOkay, I can't find it, but I have to go anyway. Class is over. Thanks so much! I have to go pick up my daughter, but we are REALLY looking forward to Thursday!
- Bugscope Teamgreat!
- Bugscope Teamcrystal, the presets will be fine for your session, they drift over time, that's why we do the presets right before your session starts
- Bugscope TeamThank You Crystal! We look forward to seeing you Thursday>
- Bugscope Teamawesome, thursday it is
- Bugscope Teamhigh noon
- Bugscope TeamOkay I think that is it Alex San.
- Bugscope Teamand by high noon i mean 1PM
- Bugscope TeamGood job, you and Cate, setting up.
- Bugscope TeamOver and out.
- Bugscope Teamsession is at 1PM CST
- Bugscope Teamyeah 1 p.m.
- Bugscope Teamcoolness, me close session now
