lycée rochambeau
Session 2007-016

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Transcript

Connected on 2007-05-23 10:00:00 from bethesda, Maryland, USA

• 10:08 am
• Bugscope Teamhi vincent!!!
• Bugscope Teamhey vincent!
• Bugscope TeamHi Vincent!
• Bugscope Teamany chance you have firefox installed on the computer you are using?
• Bugscope TeamMozilla Firefox?
• Bugscope TeamWelcome to bugscope!
• Bugscope Teami see you are using IE6, firefox would be much better.
• Guestcan it work with it?
• Bugscope Teamthe layout will be a little wonky, and you won't get the best quality streaming video, but it will work if necessary
• Guestok, thank you
• Bugscope Teamokay vincent, we just gave you control, can you try driving, or clicking a preset, let's see how it works
• Guestyes, i'll try
• Bugscope Teamdriving the scope works in two different ways: click to drive and click to center.
• Bugscope Teamgood!

• 10:14 am

• Bugscope Teamwhen you get a chance i mean.... you are doing a great job driving so far. any questions?

• Guestgood, my students are going to attempt to manipulate
• Bugscope Teamawesome!
• Bugscope Teamremember, click once to start moving, click again to stop moving.
• Bugscope Teamif you get lost, you can click on a preset to find your way back to a nice image. the presets are at the bottom right, ah you just clicked on a preset. good work!

• Bugscope TeamLooks good!
• Bugscope TeamYou could tell that was is a fruit fly eye by the setae that are sticking out from between the individual facets (the ommatidia).

• 10:19 am

• Bugscope TeamBe sure and let us know whenever you have questions about the insects or the electron microscope or anything else.
• Bugscope TeamThese are the sensory apparatus that let the fruit fly adjust the movement of the haltere so that it is coordinated with the motion of the wings.

• Bugscope TeamNow you see carbon tape, and the ant...
• Bugscope Teamone of the ants

• Bugscope Teamvincent, you are one of the BEST bugscope drivers so far!
• Bugscope TeamYou can always change the mag, refocus, change the brightness/contrast.
• Guestthank you

• Bugscope TeamThis is the space between samples on the stub. This is carbon tape and in some places silver paint to make the samples conductive.

• Bugscope TeamNow we are looking head-on at one of the beetles.

• Bugscope TeamYou can see the clubbed antennae, or you could...
• 10:24 am

• Bugscope Teamback on the tape. and back to the beetle, now the middle of the ventrum.

• Bugscope Teamlooks like a forest after a fire
• Bugscope Teamyeah!
• 10:29 am
• GuestIs it possible to save a pic?
  Bugscope TeamImages are automatically saved whenever possible and stored in the database. When your session is done we can send you a link to the transcript which will include all the images from the session

• Bugscope Teamyes, all images are saved and you can view them after the sessions, chas can tell you details
• Bugscope TeamThe pictures are all saved.
• Guest'Why the hair are broken??

• Bugscope TeamThey are brittle, and when the insect died he/she probably rolled around on his/her eyes.

• Bugscope TeamEach time you change mag, or hit a "done" button it saves an image.
• Guestok thanks
• Bugscope TeamThose setae help the fruit fly determine windspeed, from what we understand.

• Bugscope TeamThis is a closeup of the haltere.
• Bugscope TeamYou can take the mag down now to see where you are -- take it to a lower magnification, if you want.

• Bugscope TeamNow you can see more of the body of the fruit fly.

• Bugscope TeamYou can easily go still lower in mag.

• Bugscope Teameep a bit bright

• Guestyeah
• Bugscope Teamyou can use the "Adjust" tools to fix that

• Bugscope TeamGood job!
• 10:34 am

• Guestwhat are these?
• Bugscope TeamThe antennae are both broken off.

• Bugscope TeamThe dark spots are where the antennae were, both a pad-like portion and a branch-like portion.

• Bugscope TeamBut there are two more fruit flies in the vicinity that may be in better shape.

• Bugscope Teambest way to find them would be to drop down to the lowest mag and start driving
• Bugscope TeamYeah this is a claw, the end of the tarsus, and you can see the pulvillus to the right, with tenent setae on it. Oops not now though.
• Guestwhy is there an opening in the tarsus?
  Bugscope TeamI think that might have just been a cavity where the joint between the two leg sections is
• Bugscope TeamThe hole between the claw and the 'forearm?'

• Bugscope TeamIt may be that there is really an opening there, and it may be that the membrane that was once there has dried and shrunken away from its original position.

• Bugscope TeamThis is the eye of a fruit fly on the left and vestiture on the right.

• Bugscope TeamNow we are looking at some maxillary palps on a beetle's face.
• Guestis it broken?
• Bugscope Teamnow that really is a hole! looks like the arm/(palp?) got snapped
• Bugscope Teamyes, it looks broken, doesn't it. neat.
• 10:39 am
• Guestits pretty sweet

• Bugscope TeamYes you can see that it is broken. It is difficult to keep from damaging these samples.
• Bugscope TeamNice imaging!
• GuestI know. It is really sharp images
• Bugscope TeamWe are sorry not to have been able to have the ants for you -- the ones you sent. But they are still very vigorous.

• Guestno problem, this is great

• Bugscope TeamThe ants will be helpful for us in the future, maybe even when you connect with us again.
• Bugscope TeamWhere is your school?
• Guestin Bethesda, Maryland, right next to DC

• Bugscope TeamOn Whittier Blvd.
• Guestoh yes that's really near us

• 10:45 am
• Bugscope TeamYou can see that the brightness here was really high just so we could see the haltere.
• Bugscope TeamYou can see the wing to the right there.
• Bugscope TeamAnd another haltere.
• Bugscope TeamTwo-winged insects have halteres to balance the motion of the wings, like a gyroscope.
• Bugscope TeamThe halteres are heavily modified wings.
• Bugscope TeamThere I changed the brightness. Deus ex machina.
• Bugscope TeamYou can see the mouth now.
• Bugscope TeamThe background is silver paint.

• Bugscope TeamThe silver paint is on there to help carry the electrons to ground.
• Bugscope TeamThe sample is coated with gold-palladium to make it conductive.
• Bugscope TeamThe mouth is dry and not quite what it would look like were the insect alive.

• Bugscope TeamOne thing that is often a surprise when you are using an SEM, like you are today, is how 'hairy' the insects are.
• Bugscope Teammany of the hairs, which we call 'setae,' are sensory.
• Bugscope TeamWhen insects air-dry, the capillary force of the water as it recedes pulls the soft structures together causing them to shrivel.
• 10:50 am
• Bugscope TeamHere you can tune up the focus if you would like...
• Bugscope TeamOther way?
• Bugscope TeamOne of the reasons why insects are such a good choice for simple EM imaging is that their hard exoskeleton doesn't shrivel as they dry out, minimizing the amount of sample preparation required
• Bugscope TeamThere.

• Bugscope TeamIt's a knee.
• Bugscope Teamgreat! lowing the contrast will bring out some more detail on the foreground there

• Guestthis is a great image
• Bugscope TeamOh yeah. These are all going into your database of images.
• Bugscope TeamLooks like an old bicyclist's knee.
• Guesthaha

• Bugscope TeamSome of the big spines are analogous to cat or rat whiskers -- they are mechanosensory.
• Bugscope TeamVincent: If you'd like to preview what the transcript will look like so you know how images are saved, etc. you can see it here http://discord.itg.uiuc.edu/bugscope/members/2007-016/transcript
• Guestwhat role do the smaller hairs play
• Bugscope TeamThe base of the seta, or spine, is attached through the cuticle, through the chitin, to nerves.
• 10:55 am
• Bugscope TeamI am not sure about this but I think the smaller hairs give the insect more surface area and enable it to remain aloft more readily.
• Bugscope TeamTo an insect the air is thick, like water is to us.
• Bugscope TeamSome of the hairs (setae) are chemosensory. The insect can use them to detect smells --chemicals -- in the air.
• GuestYes
• GuestLike their antenae?
• Bugscope TeamYes the antennae will have lots of chemosensory setae.
• Bugscope TeamBut they also have them in other places too. Butterfly's I believe have some chemosensory hairs on their feet
• Bugscope TeamMonarch butterflies have chemosensory setae on their feet. Whoops.
• Bugscope TeamChas beat me.
• Bugscope TeamThey can land on something, scratch it, and smell the scent that is freed from the wound.
• GuestIt is noon here so we wanted to know if we could disconnect for 20 minutes and reconnect right after at 11:30 your time?
• Bugscope TeamYou can see that some of the setae here have bases that go through the chitin.
• Bugscope Teamsure, that's fine, we'll see you at 11:30 CDT
• Bugscope Teamok then
• Guestthank you that's perfect.
• Bugscope Teamsee you soon
• Bugscope TeamOkay I am running upstairs for food. See you in 30.
• 11:00 am
• Guestbon appétit!
• Bugscope Teamau voure!
• Bugscope Teamack, mispelled, sorry
• 11:32 am
• Bugscope TeamIt's not pigmented (I know I am out of private chat). It is like the colors you see on the grooves of a record.
• Bugscope Teamah, so it's a natural effect with the structure of the surface, the enviornment, and our eyes?
• Bugscope TeamThat is why I have been intrigued with scales lately, because the patterns vary so much.
• Bugscope TeamAbd we had been looking at, like, white moth scales, and yellow, etc.
• Bugscope TeamAnd..
• GuestHey Scott, I am here again with other students
• Bugscope TeamGreat! Welome back!
• Bugscope Teamhello vincent and students!
• Bugscope TeamGreat to have you back
• Bugscope TeamWe have been talking about how color is produced in the insect world, among other places. Whether the color comes from a pigment or from the shape of features on a tiny scale.
• Bugscope TeamIf there are more computers available, the students could login on those to ask questions directly

• Bugscope TeamThis is the fruit fly eye, with the facets called ommatidia, and the little sensory setae, and the vestiture off to the right.
• Bugscope TeamThe eyes of flying insects almost always seem more complex than those of terrestrial insects.
• 11:38 am

• Bugscope Teamvincent, you've driven off the edge of the sample, can you try clicking on a preset?

• Bugscope Teamokay, now we are back, yay!
• Bugscope Teamclick to start moving, then yiou MUST click to stop moving, you are forgetting to click to stop.
• Bugscope Teamclick on a preset again. i'm fixing the scope
• Bugscope Teamthere ya go

• Guestgood

• 11:43 am

• Bugscope Teamwe are off the sample again. am fixing it, click on a preset please.

• Bugscope TeamPerhaps it would be best to use click-to-center to make smaller moves around the sample, starting from one of the preset locations

• Guestwhat is it?
  Bugscope Teamwe're looking at the end of a leg. the curved part is a claw on the foot there
• Bugscope TeamoKay I am back had some consulting to do
• 11:48 am

• Bugscope Teamthere's the other of the claws on the left there too. behind it we're seeing some other body structures, can't ID them at this mag though

• Bugscope TeamSo yeah this is a claw, and the part inside that makes the claw work is a tendon-like fibrous thing called an empodium.

• Bugscope TeamAnd I am behind, now we're on the ant head.

• GuestWe are here to practice for our session next week
• Bugscope TeamYou can see the jaws, which fold open like a gate.
• Bugscope TeamOh wow Hi Cathy!
• GuestHello
• Guestok Scott, thanks for the session, it was great
• GuestThis is so cool!
• Bugscope Teamvincent you have the scope for another hour, if you like.
• GuestI hope to be able to manipulate again next year
• Bugscope TeamYou bet. We will be glad to see you.
• Bugscope Teamvincent, it was great to have you, you are welcome back next year, yes!
• GuestThank you, have a good day. My students really appreciated the session
• GuestBye
• Bugscope Teamciao
• 11:57 am

• 12:06 pm

• 12:12 pm