Connected on 2013-11-04 12:20:00 from Cheatham, Tennessee, United States
- Bugscope Team welcome to bugscope! We are venting the chamber, and in a minute we will be pumping the sample to vacuum.
- Bugscope Team oince w
- Bugscope Team once we get to vacuum, we will begin making presets
- Bugscope Team hello!
- Teacher hello. I am online
- Bugscope Team Nora we gave you control but would prefer to have you hold off on driving until we've collected all of the presets
- Bugscope Team we're going around the stub finding cool stuff for your session
- Teacher I can wait.It is not time yet.
- Bugscope Team super cool. we're glad to see you on!
- Bugscope Team this is a fruitfly
- Bugscope Team Nora we are ready, if you'd like to drive, look around
- Bugscope Team oops we need to remake some of the presets...
- Teacher just a minute more
- Teacher ok. ready
- Bugscope Team so Nora is your class there?
- Bugscope Team please let us know when anyone has any questions
- Teacher yes
- Bugscope Team we're still putting in presets, but we are essentially ready
- Teacher ok
- Teacher whatismit?
- Teacher what is it?
- Teacher is this the fruit fly?
- Bugscope Team the thing we just saw that looked kind of like a corncob was a brochosome, from a leafhopper -- an extra long one. they use them, we think, to help keep their eggs from drying out
- Bugscope Team now we're totally done making presets
- Bugscope Team this, right now is a moth scale
- Bugscope Team moths, butterflies, silverfish, and mosquitoes have scales like this
- Bugscope Team this is a loose one, on the grasshopper
- Bugscope Team scales come off easily, and thus if you were covered with scales and flew into a spiderweb, you have a good chance of leaving your scales stuck to the web and escaping
- Bugscope Team to the lower left we see one of the grasshopper's claws
- Bugscope Team pigment granules and the way the scales are structured are responsible for the colours you see in moths and butterflies
- Teacher ok
- Teacher next?
- Bugscope Team this is a bunch of plant material and perhaps some fungal hyphae -- strands of fungus -- on the grasshopper
- Bugscope Team please note that you can click on any of the presets, to the left -- on the lefthand screen, in order to get the microscope to drive to that place.
- Bugscope Team on the grasshopper's leg, you can see lots of hairs
Bugscope Team these hairs are called setae, and they help the grasshopper sense their environment
Bugscope Team the scale you just saw is a modified type of setae
- Bugscope Team so I just clicked on the cranelfy preset
- Teacher eyes?
Bugscope Team yup
- Teacher southern cranefly?
- Bugscope Team this one is local to Illinois -- they look like giant mosquitoes, but they are harmless and kind of clumsy
- Bugscope Team im not sure where this cranefly is from. it is possible it was captured locally around here (central Illinois)
- Bugscope Team the background with all of the little craters in it is carbon tape we stick the insects to
- Teacher too large
- Bugscope Team and also, to the lower right, we see silver paint, which helps the insects stick and also lets the charge from the electron beam travel to ground
- Bugscope Team it is a little tricky driving the microscope
- Bugscope Team this is the crane fly, here you can see its mouth parts pointing up
Bugscope Team Many crane fly adults, do not feed, and when they do, they feed on nectar
Bugscope Team Most larvae feed on plant materials, and feed on plant roots
Bugscope Team Some are carnivorous and will eat other arthropods
- Bugscope Team you can try the magnification - button in the top left area of the image
- Teacher how do i minimize?
Bugscope Team to take the microscope to the lowest magnification, you can click on the minus button at the top of the window, after 'Magnification.'
- Bugscope Team the scalebar on the lower left gives us a sense of the magnification
- Bugscope Team you can see the beetle's compound eyes, on either side of its head, and all of its mouthparts, including four palps that help it taste and manipulate its food
- Teacher next?
- Bugscope Team you can also see its antennae
- Bugscope Team let's go to the stinkbug, kind of cool
- Bugscope Team here is part of the stink gland on a stinkbug
- Bugscope Team stinkbugs do not like their own smell
- Bugscope Team it also has some spongy parts that help with sucking up extra stink so the smell doesn't bother them
- Bugscope Team you can see the compound eyes fairly well here.
Bugscope Team the compound eye is composed of individual units called ommatidium, each of these units collect a part of the whole image, and they work together to provide the insect with a complete view
Bugscope Team the complete image ends up being a mosaic like image
- Bugscope Team so they have all of those absorbent structures, to the upper left, that help keep it from bothering the stinkbug
- Bugscope Team you can see that the micron bar now reads '50 microns,' which is about 25 bacteria long
- Bugscope Team so if you wanted you can take this to a lower magnification to see where you are on the ventral side (the underside) of the stinkbug
- Bugscope Team Many stink bugs are considered to be pests. Most feed on plants.
- Bugscope Team the stink gland opening is on the underside of the body -- of the thorax -- between the first and second set of limbs
- Bugscope Team so its tiny claw?
- Bugscope Team we can tell that its head is to the northeast
- Bugscope Team Some countries eat them, and the distinct smell/taste is considered to be a delicacy.
- Bugscope Team cool! now we can see one of the compound eyes
- Bugscope Team and we can see the base of the piercing/sucking mouthparts that make the stinkbug a 'true bug'
- Bugscope Team everything we see is in black and white -- in grayscale -- because we are using electrons rather than light to image our specimens
- Teacher do you have any spidersor corpions?
Bugscope Team no i dont think we have any spiders, and definitely not scorpions. we don't come across too many of those in illinois
- Bugscope Team the images we see come to us as signal
- Bugscope Team you are driving a $600,000 scanning electron microscope from your classroom
- Teacher I can send you some:)
Bugscope Team that would be so cool!
- Bugscope Team this is a female housefly
- Bugscope Team we're looking at the top of the head
- Bugscope Team you can see its streamlined compound eyes
- Bugscope Team and you can see, perhaps, the tiny simple eyes called ocelli
- Bugscope Team now we're way up high on some of the facets of the compound eye
- Bugscope Team this is pretty
- Teacher pollen on the eyes?
Bugscope Team those are super small -- they look like pollen but are much smaller, on the nanoscale
- Bugscope Team they are brochosomes as well -- usually they are round like this
- Bugscope Team brochosomes are about 250 to 400 nanometers in diameter
- Bugscope Team so they are smaller than the wavelengths of visible light
- Teacher next..
- Teacher what are they?
Bugscope Team they are tiny structures said to be produced in the Malpighian tubules of leafhoppers.
- Teacher my students would like to move a little faster, please
- Bugscope Team this is a pollen grain
- Bugscope Team it's spikey so it can stick to insects and thus travel around
- Bugscope Team we can see that it is larger than the brochosomes
- Bugscope Team this is the grasshopper head
- Bugscope Team you can see its antennae, and its mouthparts, and its compound eyes
- Bugscope Team large and bulbous
- Bugscope Team feel free to select a preset located on the left (click on the blue and white arrow button first) to change the area
- Bugscope Team this is a closeup of a scale, so cool
- Bugscope Team and this is a palp; they often have things like tastebuds inside them, or at their tips
- Bugscope Team these are setae, very beautiful, on the silverfish
- Bugscope Team in some butterflies, the taste buds are actually in their forelegs!
- Bugscope Team silverfish are mostly in the dark, and they have to be wary of spiders
- Bugscope Team so we can see that the silverfish has scales like a butterfly or moth or mosquito
- Bugscope Team the ridges reflect silver light
- Bugscope Team which is why silverfish look silvery
- Bugscope Team these guys can be pretty devastating for a library, they feed on the starches and other poly saccharides in book bindings
Bugscope Team they are also able to live for a long time without feeding
- Bugscope Team there are the fruit flies we were looking for
- Bugscope Team you can see the compound eyes, and the very small antennae
- Bugscope Team amd also one of the arms, with all of the tarsal segments
- Teacher be right back in 7 minutes
- Bugscope Team see the claw?
- Student we're back
- Student new class
- Bugscope Team we're looking at a beetle palp
- Bugscope Team if we take the magnification down, we can see just where we are
- Bugscope Team do you want to try that?
- Student yes
- Bugscope Team now we see one of the eyes, to the right
- Bugscope Team looks like a golfball
- Bugscope Team do you see where we were, now? on the right?
- Bugscope Team this beetle's head is about 1 mm wide
- Student what is the tooth looking appendage?
- Bugscope Team in the front of the head, the big flat thing is called a clypeus -- one of the mouthparts
- Bugscope Team below the clypeus is one of the mandibles
- Bugscope Team insect mouthparts usually open left and right, instead of up and down like ours
- Bugscope Team the clypeus slides up and down, though, and then the mandibles open to the left and right like a gate
- Bugscope Team They use the mandibles to break hard things, seeds, other insects' cuticles, etc...
- Bugscope Team flies do not have the same kinds of mouthparts
- Bugscope Team This is a fly, you can see it's antennae right above the eyes (the two twinkie looking like things)
- Bugscope Team the antennae have a twinkie part and a branched part called an arista
- Student what about biting flies
Bugscope Team they have slashing/cutting mouthparts, and we do not see them as often, thankfully
- Bugscope Team horseflies and deerflies, for example, cut the skin to make blood flow and then drink that
- Bugscope Team this funny-looking dude is a silverfish
- Bugscope Team they eat the glue that holds your books together
- Bugscope Team we can see that it has a clypeus like the beetle, and you can see a tiny forked mandible beneath that
- Bugscope Team insect mouths are often very complicated
- Bugscope Team we can see that one of the silverfish's antennae is broken
- Bugscope Team this is another kind of fly
- Bugscope Team a cranefly
- Bugscope Team you can see its compound eyes, with many tiny facets called ommatidia -- individual lenses
- Bugscope Team flies often have sponging mouthparts -- they soak up food in liquid form
Bugscope Team some more 'primitive' flies have chewing mouth parts still, and mosquitoes and some other biting flies have a specialized straw like mouthpart
Bugscope Team sorry, i think I am wrong here, I was thinking about butterflies and moths (in regards to chewing mouth parts)
- Bugscope Team these kind of look like big mosquitos
- Bugscope Team the scanning electron microscope can help us collect images at much higher magnfications than light microscopes
- Student is that an eye?
Bugscope Team yes these are the ommatidia of a compound eye
- Student ok
- Bugscope Team the ommatidia are shriveled, a bit
- Student next please
- Bugscope Team some wasps have as many as 30,000 ommatidia per eye
- Teacher Cool
- Bugscope Team insects do not breathe through their mouths
- Bugscope Team they have these pore-like things called spiracles
- Bugscope Team they can open and close them
- Bugscope Team there are two spiracles on either side of a body segment, usually\
- Teacher What is this called?
Bugscope Team that was one of the thoracic spiracles on the fly body
- Bugscope Team now we're looking at mold spores
- Bugscope Team they look much like pollen but are generally softer and have smaller spikes
- Bugscope Team if we take the mag down we can see where we are
- Bugscope Team in the foreground, to the left, we see one of the palps that grasshoppers have, with a flat opening
- Student ok
- Teacher What do they use them for
- Bugscope Team palps help insects taste and also manipulate their food
- Teacher Ok
- Bugscope Team now we see that we were in a tiny valley on the grasshopper's head, where the mold spores were
- Student ok
- Bugscope Team the grasshopper seems to have an extra eye in the middle of its forehead, between the antennae
- Bugscope Team compound eyes help insects see better -- all around them -- without moving their heads
- Bugscope Team also, if you had compound eyes
- Bugscope Team you would be able to see movement
- Bugscope Team more quickly
- Teacher So they can see every side of there body with them kind of eyes
Bugscope Team yes! it is super helpful in the insect world, where things move so very quickly
- Bugscope Team Bruce you have the same name as the Hulk.
- Bugscope Team The extra eye is called and ocellus, it is used to differentiate light and dark, and aids in flight and also circadian rhythm (body's internal clock)
- Teacher I know.. Hahaha
- Bugscope Team this is one of the beetle's claws
- Bugscope Team we can see the sticky hairs, called tenent setae, that help the beetle climb walls
- Teacher How many claws do they have???
Bugscope Team they have six sets of claws -- one set of claws per limb
- Teacher Do the not have peripheral vision?
Bugscope Team they have better peripheral vision than we do
Bugscope Team some insects (like the dragonfly) can see 360 degrees around
- Teacher Can they hurt you with their claws?
Bugscope Team no they are so small we can hardly feel them
- Bugscope Team not all claws have double tips like that
- Bugscope Team some claws open and close using an internal tendon called an unguitractor
- Teacher Do they ever use them for defense or to kill prey?
Bugscope Team I'm sure they do sometimes
- Bugscope Team praying mantids have little teeth-like parts on their legs that help keep their prey in place
- Bugscope Team you know sometimes we see beetles that cannot climb, but clearly these can, no problem
- Bugscope Team this is the tip of one of the super tiny tenent setae
- Bugscope Team 2 microns, on the scalebar at the bottom left of the image, is as long as a bacterium
- Bugscope Team those setae (hairs) are called microsetae; they are not sensory like setae
- Teacher What is on the tips of the hair that makes them stay on a wall
Bugscope Team sometimes it is a fluid, and sometimes it is just the shape and the fact that there are many of them
- Bugscope Team the ridges we see now, on this moth scale, are so small that they interfere with the wavelengths of visible light and produce colors just from their shape
- Bugscope Team I want to see if we can find some brochosomes
- Bugscope Team this is the head of a female housefly
- Bugscope Team here are three extra eyes, simple eyes called ocelli
- Bugscope Team these are the ocelli, and we see setae and microsetae
- Bugscope Team awesome
- Student break time..5 minutes please..
- Teacher Bye you're awesome!!!!!!!!!!!!!!!!!!!!
Bugscope Team thank you Huff!
- Teacher We love you!
Bugscope Team sweet
- Teacher By thanks for the experience
Bugscope Team bye Hulk!
- Bugscope Team now we see brochosomes on a ragweed pollen grain
Bugscope Team brochosomes serve a protective function in leafhoppers, waterproofing and excrement-proofing
- Bugscope Team this is the head of a small beetle
- Student we are back..
Bugscope Team welcome back@
- Student ok
- Guest What does this do?
- Student hello?
- Guest How do I use this?
- Bugscope Team here I will show you again
- Bugscope Team we are using electrons to image. electrons hit the sample and secondary electrons come back from the metal coating. the secondary electrons are gathered in a detector, where it reads the signal from them and gives us this image
- Bugscope Team there is a camera inside the chamber, but you don't see it when imaging
- Student this is the camera?
Bugscope Team this is the inside of the chamber, where the electron beam is impinging on the sample
- Guest How do I see the image on my phone
Bugscope Team um go to Bugscope and log in from there; is that what you mean?
- Guest What is this bug?
Bugscope Team this looks like a little longhorned beetle
- Guest All I have is the chat
Bugscope Team I'm sorry -- when you get to a larger computer you can go to the webpage and look at archived images. I'll try with my phone and see what happens.
- Guest Okay thank you I appreciate it
Bugscope Team JenkaDink43 I am sorry -- I get the same as you, no images.
- Student next
- Guest Okay it is fine I was just curious to see if I could show others what we were learning today
- Bugscope Team this is the head of a hsol\\
- Bugscope Team housefly
- Bugscope Team now we see its antennae and its compound eye, to the lower portion of the image
- Bugscope Team there are some plant fibers on the eye, to the left
- Bugscope Team this is where the mouthparts tuck into the front of the face
- Bugscope Team here is another housefly head, another female housefly
- Bugscope Team when we were looking at it earlier we found a pollen grain with nanoparticles on it
- Bugscope Team here is the compound eye, to the left, with hundreds and thousands of ommatidia
- Guest What do they use the hairs for above their eyes?
Bugscope Team they are mostly for sense of touch.
- Bugscope Team to the far right we see two ocelli -- simple eyes
- Bugscope Team we're looking from a funny angle, from the top of the head
- Bugscope Team the tiny hairlike things are called microsetae, and they form the vestiture - the dressing of the head
- Guest The curved hair like structure above the eye what is it for?
- Bugscope Team that is likely a mechanosensory sete
- Bugscope Team seta, sorry
- Bugscope Team yes the big hairs are more for sense of touch, or mechanosensory like sj said
- Bugscope Team this is a pollen grain, we think ragweed pollen
- Bugscope Team among the microsetae
- Bugscope Team smaller hairs are often for sense of touch, or other things like smell/taste, temperature, etc
- Bugscope Team see the little soccerball like things?
- Student yes
- Bugscope Team those are brochosomes, which are produced by leafhoppers but get onto a lot of other insects
- Bugscope Team they are nanoparticles, 250 to 400 nm in diameter
- Guest So basically it's like the nerves in our fingers that can feel things and sense temperature
Bugscope Team yes exactically insects do not have skin so they need to have setae that stick through the cuticle so they can sense their environment
- Guest Oh I gotcha
Bugscope Team insects and comparable arthropods -- it's like they're wearing a suit of armor all of the time
- Bugscope Team this is a silverfish
- Bugscope Team you can see that the silverfish is covered with tiny rounded scales
- Student yes
- Guest Why do you call it a silverfish? If it's not a fish?
Bugscope Team it's silver in color and when it runs it kind of squirms like a fish
- Guest Oh I understand
- Bugscope Team they're like potato chips
- Bugscope Team it also has scales but not like a fish, i don't think it has anything to do with why they called it that
- Bugscope Team moths, butterflies, mosquitoes, silverfish, and few other insects have scales, which protect them from getting caught in spiderwebs
- Bugscope Team I think it's 'cause they are silvery and kind of slimy the way they move, like a small fish
- Student next
- Guest Oh I understand thank you
- Bugscope Team we can see its compound eyes, and also its mouthparts, including its palps, which in grasshoppers have hollow openings
- Bugscope Team this grasshopper has an extra eye in its forehead
- Student why
- Bugscope Team i think it helps with balance by seeing where the sun is in the sky
- Bugscope Team usually simple eyes like this help orient insects with the sky, with the sun and other features so they don't get lost
- Guest Is it to see things more clear coming from above? To help avoid predators?
Bugscope Team they use the compound eyes, which produce better images, to see predators
- Student ok
- Bugscope Team compound eyes also give the insect better peripheral vision as well as being more sensitive to motion
- Guest Sorry my phone is a bit slow sending in my questions or responses
Bugscope Team we're happy to be working with you
- Bugscope Team see the mold spore on the eye?
- Student what is the elongated structure?
Bugscope Team some of those things we don't know what they are
- Bugscope Team the little spines probably help the grasshopper feel things touching its head
- Bugscope Team the elongated structure here looks like a broken off seta with some juju coating it
- Guest Why does Duru consist of being a little bitch?
- Bugscope Team this is a cranefly
- Student please excuse my infantile students
- Bugscope Team looks like a very large mosquito
- Guest I'm sorry that was not actually me
- Bugscope Team it's ok
- Bugscope Team we can see its compound eyes quite clearly
- Student what is that?
- Bugscope Team crystals
- Bugscope Team super small crystals of sugar, perhaps
- Student crystals on the eye
- Student sugar on the cranefly
- Bugscope Team not a problem. really
- Guest I appreciate everything you have done for us and have a nice day my apologies again
Bugscope Team we had fun, no worries
- Bugscope Team oh and we do not know what this is
Bugscope Team it's a clump of pollen grains all stuck together
- Bugscope Team time for us to shut down and let the researchers get back in
- Student thank you so much. they are gone. it was great. a litle slow.
- Bugscope Team Thank you, Nora!
- Student I think the third show was the best of the 3
- Bugscope Team See you next year!
- Bugscope Team thank you for using bugscope with us todau
- Bugscope Team today*
- Student sorry about the profanity
- Student my classes are all jocks and all male
- Bugscope Team all good
- Student bye
- Bugscope Team Bye!