Connected on 2008-04-17 10:00:00 from Urbana, Valle del Cauca, CO
- Bugscope Team hello nancy
- Bugscope Team Hi Nancy
- Bugscope Team where are you from?
- Guest hi, tucson az
- Bugscope Team hola juan
- Bugscope Team Things are pretty quiet right this moment, we're waiting for a live connection from Columbia. Ah, they just arrived
- Guest hi..a little late
- Bugscope Team Hola!
- Bugscope Team Hi MenDuGo!
- Guest hi how are you_
- Bugscope Team Good!
- Bugscope Team Juan!
- Teacher hi again
- Bugscope Team Hola, bienvenidos a bugscope
- Bugscope Team Bienvenidos.
- Guest Thanks!
- Teacher Gracias.
- Bugscope Team hola marval
- Bugscope Team hola marval
- Student hello!!!!!
- Bugscope Team You can see on the screen where the microscope has been imaging for a long time today.
- Bugscope Team We burned a mark on this eye.
- Teacher the guys are just gettin logged..give us a minute
- Student hello
- Bugscope Team no problemo
- Guest what objects we are observing
- Bugscope Team Juan you have control, and if things do not seem to work for you let us know. And take your time.
- Student hi Cate!!
- Bugscope Team this image is a close up of a compund eye
- Bugscope Team compound eye i mean
- Student holaa
- Student what are we watching?
- Bugscope Team MenDuGo this is a bugscope connection with a class in Colombia.
- Bugscope Team these are images from an ESEM (environmental scanning electron microscope)
- Bugscope Team this is the compound eye of an insect, I think a true bug, but I don't remember right now. Someone would have to take the magnification down a little
- Guest I know Scott I am a student of Juan
- Bugscope Team descachaladrada, that is a long name, but once you get the hang of it it's nice!
- Student duarte
- Bugscope Team me llamo alejandro
- Bugscope Team Cool!]
- Student and now??
- Bugscope Team Oops.
- Student santi how are you
- Bugscope Team So MenDuGo totally cool.
- Bugscope Team a limb is covering part of the head
- Teacher Hi!!!
- Bugscope Team hola juan
- Bugscope Team We are concerned that there will be a big lag time when you actually drive the 'scope. So let us know if we need to drive or can help in any way from here.
- Bugscope Team very nice to see you again, you should have control now
- Bugscope Team Juan has control of the 'scope now.
- Bugscope Team This is a 'true' bug.
- Bugscope Team yes, juan, if your lag is bad, then please let us know, we can drive for you, and show you interesting things, and tell you about them.
- Teacher what is the reason why som students are getting logged out automatically??
Bugscope Team we do not know of anything that would do that on our end
- Bugscope Team if any of you have questions, please just ask
- Student juan lets go and do it
- Bugscope Team There are two true bugs on the sample stage and some other insects as well.
- Guest Hi again, can you tell me what type of insect it is?
Bugscope Team This looks like what we call a plant bug
Bugscope Team this is a true bug. True bugs have piercing mouthparts.
- Bugscope Team But it happens sometimes.
- Bugscope Team This is a hemipteran.
- Student what type of insect is that
- Student what is this?
- Guest hoo cool
- Student Hi!!
- Student whats that?
- Bugscope Team juan, maybe the limited network bandwidth is causing some students to be logged out or disconnected. maybe double up students on a computer will help?
- Student A bug
- Bugscope Team They have piercing mouthparts and a particular orientation of their wings that characterize them.
- Teacher what degree do you guys have?
Bugscope Team I have a masters degree in entomology and I am working on my PhD
- Bugscope Team of their wings/elytra.
- Guest what is the common name of insect_
- Bugscope Team various degrees
- Student Whats the name of the insect we are looking at?
Bugscope Team I believe this is a plant bug, an insect in the family Miridae.
- Student what is the real size of the byg
- Bugscope Team i have a master degree in music composition
- Student what is the real size of the insect
Bugscope Team well, the insects are anywhere from millimeters to centimeters, if you zoom out, you can see the whole insects, and then look at the scale bar in the lower left of the image for scale
- Bugscope Team I have a bachelor degree in Physics
- Bugscope Team you can get an idea of the size from the scale bar on the lower left side of the screen.
- Student what is the common name of that bug
Bugscope Team I think it is a plant bug
- Student What is the real size of the insect?
- Bugscope Team not sure of the common name
- Student which is the scientific name?
Bugscope Team I am not sure of the species...I study beetles--which are hard enough!
- Student What have you studied?
Bugscope Team I study the evolution of pheromone communication in longhorned beetles.
- Bugscope Team it is probably a little more than a cm long
- Bugscope Team I have a degree in English and Biology and have been doing electron microscopy since I got out of college 25 years ago.
- Guest lol
- Bugscope Team please try clicking on one of the presets when you are ready. We are anxious to see how much control you have.
- Student with what kind of electronmicroscope are we watching this insect?
- Bugscope Team So far it looks good.
- Bugscope Team if you want to go to a different insect/object, you can click on a preset in the lower right corner
- Bugscope Team This is a field emission scanning electron microscope.
- Bugscope Team this is an environmental scanning electron microscope (ESEM), here is a picture of it: http://www.itg.uiuc.edu/ms/equipment/microscopes/esem/
- Teacher are we going to see any other insects?
- Bugscope Team it is actually an environmental scanning electron microscope, but we are using it in HiVac mode now.
- Student what kind of special characteristics it has?
Bugscope Team check out this webpage for information on this scope: http://www.itg.uiuc.edu/ms/equipment/microscopes/esem/
- Guest Ok, I have some quiestions: What is the common name of the Hemipteran?
Bugscope Team The common name for insects in the order Hemiptera is true bugs
- Bugscope Team if you want to see another insect have Juan click on one of the presets, to the right of this chat box on his computer.
- Bugscope Team if that does not work let us know
- Bugscope Team cool looks like it is working
- Teacher ok..im changing the view to another insect
- Bugscope Team juan is controlling the scope now. we can give control to any other student at any time. but onle one person at a time
- Bugscope Team you can see the eyes take up most of the area of the head
- Student Do you have a general picture of the microscope?
Bugscope Team yes, there is a picture of it on this website: http://www.itg.uiuc.edu/ms/equipment/microscopes/esem/
- Student what is the name of this insect
Bugscope Team this is just a small moth. I dont know the particular species
Bugscope Team This is a moth
- Bugscope Team the proboscis (its mouthpart) is curled up in the middle of the head
- Student what is this?
- Bugscope Team this microscope has backscattered electron detectors, energy-dispersive spectroscopy (elemental analysis), a Peltier heating/cooling stage, electrical feedthroughs, two hot stages, etc.
- Student A fly
- Bugscope Team you can see now that this is several mm long.
- Bugscope Team this is a moth -- you can tell by the scales
- Bugscope Team a moth is a couple of millimeters, i think, or maybe bigger?
- Student Why the image is white and black and not colored?
Bugscope Team these images are from an electron microscope, and the images are gathered NOT with light, but with electrons, so it's a totally different way of accessing an image and thus we only see shares of grey, not colors
- Bugscope Team we are very happy to be connecting with Colombia. This is the first time we have connected with the southern hemisphere.
- Teacher Can I change the position of the image? (dispace the cebter?)
Bugscope Team Yes, go ahead and try the Click to Center tool in the controls on the right
- Student what are that little things in the mouth
Bugscope Team Those are scales that have rubbed off of the moth's body. Moths and many butterflies are completely covered with scales.
- Bugscope Team the image is produced by collecting secondary electrons from a sample that has been coated with gold-palladium.
- Teacher we're happy to paticipate on this project to...\
- Teacher too..
- Bugscope Team oops we did connect with Australia, but never South America.
- Student OK, thanks
- Bugscope Team yes, you are doing great juan! keep going!
- Student thank you annie
- Bugscope Team you are welcome!
- Bugscope Team the samples are in a vacuum chamber, and we beam electrons at them.
- Teacher I still can't find a way to move the picture
- Bugscope Team juan - try click to center
- Student annie what did you study
- Student how much eyes do this insect has?
- Bugscope Team juan - there is probably too much lag to "click to drive", so use "click to center" instead
- Student can you give us specific characteristics of this bug please
- Teacher ok click to drive is the option..got it
- Student how many guest do you have per day?
Bugscope Team We are a research facility, so students at the University here come to use it all day. We typically do 2-3 remote bugscope sessions a week though
- Bugscope Team click to drive will be slow because of lag
- Bugscope Team the electron beam passes continuously across the surface of the sample, and as it does it produces secondary electrons that are pulled toward a photomultiplier tube -- a PMT -- that gives us the assembled image.
- Bugscope Team try click to center instead
- Student annie, what did you study and what specializations do you have
Bugscope Team I am a PhD student in entomology. I got my master's degree in 2005 and will hopefully finish my PhD next spring. I study pheromones in longhorned beetles.
- Bugscope Team 92 um is 92 microns, or 92 micrometers
- Bugscope Team the u should be a Greek mu.
- Student annie what did you study
Bugscope Team Annie has been, and is studying Entomology -- a.k.a. the study of insects
- Teacher ok..i'll let it in click to center...
- Bugscope Team for reference, a bacillus is normally 2 microns in length
- Bugscope Team juan - you can also click on a preset (lower right side) and then the scope will move to that preset
- Bugscope Team with click to center, you should be able to click somewhere on the image and the software will make that the center of the screen
- Student In which part of the insect are we?
Bugscope Team this is the head
- Student Annieeeeeee
Bugscope Team ;)
- Student What is the high zoom the microscope can raise?
Bugscope Team the highest possible, is 600,000x, but with insects, you can't see much past 20,000x or 30,000x
- Student heyy how much eyes do this insect has?
Bugscope Team the eye is called a compound eye, which is made up of individual facets, or ommatidia
- Bugscope Team cediel - this is the eye of a moth, a compound eye
- Bugscope Team most of these are airdried and coated with 7 or 8 nm of gold-palladium to make them conductive.
- Bugscope Team this is the head of the moth
- Bugscope Team and we are looking at a few ommatidia -- the facets of the compound eye
- Bugscope Team I am going to try to remember to collect lots of interesting insects for bugscope when I am out in the field this summer.
- Bugscope Team you can see from the scalebar that we are at a fairly high magnification now, for insects
- Student scott what did you study and what are your specializations
- Bugscope Team now you can see the scalebar says 720 nanometers
- Bugscope Team wow, 77,000x, nice
- Student what are the basic advantages of the microscope youre using?
Bugscope Team Light microscopes are fundamentally limited in their resolving power (resolution) by the wavelength of the light you use, about 300-700nm. Electron microscopes use much smaller particles and so they can magnify about 100x greater than light microscopes. Also, because the electrons don't penetrate the surface, you get exceptional surface detail even on things that appear transparent
- Bugscope Team 720 nanometers is approximately the width of the wavelength of red light, so this detail would be impossible to observe using a light microscope
- Bugscope Team wow, 155,000x!
- Bugscope Team I worked with amphibian pigment cells for 5 years, mostly doing TEM. Then I was at a dental school for 10 years and looked at teeth, and bacteria, bone, implants, lots of stuff.
- Student How many zoom are we using at this momment?
Bugscope Team check out the number in the upper right, magnify, it says 155,000x, now 77,000x
- Teacher which insect has been the most difficult to analyze??
- Bugscope Team normally when we want to go to very high mag we at closer to the sample and the image will look better
- Student At which temperature does the microscope need to be, and what is its size?
Bugscope Team The microscope is generally at room temperature, and itself is about the size of a mini refridgerator, but fills a room with supporting equipment
- Bugscope Team for bugscope we have the sample at a long working distance
- Bugscope Team the microscope is now at room temperature
- Student what are the basic advantages of the microscope youre using?
- Student what parts of the bug are we looking at
Bugscope Team We are looking at the eye
- Bugscope Team when we work in wet mode sometimes we work at 4 C
- Teacher How can you control the intensity of the beam (and what effect does it have over the image?)?
Bugscope Team The shape of the electron beam can be controlled using magnetic fields, because *moving* charged particles feel a force from magnetic fields. This allows us to focus the electron beam on the sample and improve resolution.
Bugscope Team Essentially, we're using electromagnets to shape the electron beam the way a light microscope uses glass lenses to shape an optical beam. We don't often vary the intensity of the beam, a.k.a. the spot size, except in extreme situations where we want to generate a lot of X-rays for atomic composition analysis
- Bugscope Team the microscope uses liquid nitrogen
Bugscope Team The liquid nitrogen is used to cool an extra X-ray detector
- Bugscope Team this microscope will allow us to work without making the sample conductive
- Bugscope Team scott is always scaring me by pouring liquid nitrogen by my feet
- Student what parts of the bug can we see now
Bugscope Team We are still looking at the eye of the moth
- Bugscope Team Descach this is still just a few eye facets
- Teacher nice question juan
- Student How much time do we still have?
- Bugscope Team Now we are looking at a fly mouth
- Bugscope Team now we see the proboscis of a fruit fly
- Student what is this?
- Guest what is that
- Student what is the maximun size of a the sample you use in a microscope?
- Bugscope Team the stage is 50 mm across
- Guest mosca de la fruta
- Bugscope Team that's right mosca de la fruta
- Guest yes i knoe annie
- Bugscope Team this is part of the feeding apparatus that normally does not look good because (normally) the samples are air-dried
- Student bye! thanks for all
- Guest thank you good bye
- Bugscope Team but Cate critical point dried this sample for oyu
- Bugscope Team you
- Student thank you annie, scott, chasfo, chas , cate and alex!!!
- Student OK thak you very much
- Teacher ok..this is the first group..they're leaving now..thank you so much...theres another group coming
- Bugscope Team Thank you all very much for your questions
- Bugscope Team thank you students from cali colombia!
- Student We are very happy to have the opportunitie to interact with you
- Bugscope Team Great questions!
- Guest re waiting for you in Colombia pa que se la goce
- Bugscope Team yes thank you
- Student Hope you come here to visit us
- Student :D
- Guest a la orden
- Bugscope Team hasta luego!
- Guest nos vemos en diciembre en los united
- Teacher bye gyus
- Bugscope Team bye
- Bugscope Team you are coming to the US in December?
- Bugscope Team you are coming to the US in december?
- Bugscope Team nice Alex
- Guest yes
- Bugscope Team cool!
- Teacher get me some nice girls
- Student on june
- Student to LA
- Bugscope Team well we are in Illinois, south of Chicag
- Student my cousin is Pamela Rueda, she is a Biologist
- Bugscope Team Chicago
- Bugscope Team Hey Cool.
- Student and his housband discovered a new specie of fish
- Bugscope Team you dont want to go to Illinois, especially in the summer. It's all humid and sticky :p
- Bugscope Team como se dice June? Juno?
- Teacher bye thank u for everything!!!!!!!
- Bugscope Team junio
- Student Junio
- Bugscope Team Oops Junio got it, gracias
- Student OK bye and thank you very much for all
- Student ]have a nice day
- Bugscope Team gracias!
- Bugscope Team buenas dias
- Bugscope Team You too Thank You!
- Bugscope Team So Juan you seem to be able to control the 'scope well.
- Bugscope Team juan you did well driving, do you have any questions?
- Teacher I'm organizing the other group..so far so good..:)
- Bugscope Team cool
- Bugscope Team una hora mas
- Bugscope Team you are doing a great job controlling the scope.
- Bugscope Team juan, keep in mind, all the images and chat are saved on your member webpage: http://bugscope.beckman.uiuc.edu/members/2008-023
- Bugscope Team uh oh
- Bugscope Team he''ll be back
- Bugscope Team hola JD
- Bugscope Team hola Kiko
- Bugscope Team like the hasta la vista dude
- Bugscope Team hola terrozco
- Bugscope Team hola juan!
- Teacher hola caracoles
- Teacher hola tema
- Teacher hola alex
- Teacher team
- Student hello
- Bugscope Team do you say equipo for team?
- Teacher que tamano tiene el insecto?
- Student yes
- Teacher que tamaÑo tiene esa mosca
- Bugscope Team tamano?
- Bugscope Team this is a fruit fly
- Student julicar
- Bugscope Team what ---- has the insect?
- Teacher what size?
- Student hola chahin
- Bugscope Team mosca de la fruta
- Bugscope Team this is less than a cm long
- Teacher is it female o male?
Bugscope Team this is female
- Bugscope Team notice the scale bar in the lower left of the image
- Teacher we have an issue here...i have logged in again under the nick Juan_Carlos
- Teacher what size is the fruit fly?
Bugscope Team it is less than a centimeter big
- Bugscope Team it is okay juan, we know who you are as long as you log in as teacher
- Teacher i am mer nerd
- Bugscope Team Juan because you could not use your name again? that happens to us
- Teacher the nicks are all messed up...is that normal?
- Teacher i love to study
- Bugscope Team Juan this is a nerd paradise, here where we work
- Student how much zoom does the microscope has?
- Teacher what kind of insect is that
- Bugscope Team fruit fly
- Bugscope Team this is a fruit fly
- Bugscope Team it eats the fungi that is made on rotting fruit
- Teacher what did you study?
- Bugscope Team we can go over 800,000x but for publishable images usually no more than 200,000x
- Bugscope Team I studied English and Biology.
- Bugscope Team i studied music composition in college
- Student hi scott
- Bugscope Team i studies Physics
- Student hi scott
- Bugscope Team I quizzed out of Spanish but it was long ago
- Teacher I want to see another insect!!!
Bugscope Team go ahead and click on a preset to move to another insect
- Bugscope Team it is black and white because we are not using light -- we are using electrons
- Student HMM..DO YOU GUYS LIKE YOUR JOBS??
- Bugscope Team Hola SanManu
- Bugscope Team we like what we do
- Student why is in black and white
Bugscope Team instead of using light to gather the image, the ESEM uses electrons, so the image is gathered in degrees of grey, not color
- Teacher is there another machine better to watch insects?
- Bugscope Team i love my job. i am always learning something new
- Bugscope Team alex - all yours, I can't type fast enough
- Teacher how are you out there
- Bugscope Team we are privileged to be able to use electron microscopes all day
- Teacher whatb is the real size of the insect? i really love this
- Teacher how are the eyes of the fly?
- Bugscope Team because we are using electrons rather than light, we do not see colors
- Student but... are you always doing this?
- Bugscope Team the electron beam is smaller than the wavelengths of visible light
- Bugscope Team we dont always do bugscope. We have around 3 sessions of bugscope a week
- Student how is the structure of the fly
- Student animal
- Bugscope Team this is a microscopy laboratory and we train people to use all kinds of microscopes
- Student scott: how does an electron microscope work?
- Student what is the structure?
- Teacher why if you studied music are you doibg this alex?
Bugscope Team because being a computer person pays the bills much better than music does
- Bugscope Team when we see eyes that are broken, sometimes, it appears that they are actually crystalline like glass lenses
- Bugscope Team the compound eyes have individual parts called ommatidia and they each get a part of an image of whats around them and send it back to their brain
- Bugscope Team the samples are in a vacuum, and we beam primary electrons at the samples, with a great deal of control
- Bugscope Team focus!
- Student how is liquid nitrogen useful during the process?
Bugscope Team liquid nitrogen is used to cool down the EDAX, which is a separate device that can sense chemical composition of what is in the scope
- Bugscope Team the ocelli are found on top of their head so we don't always see them. They act like a compass
- Bugscope Team what we get back are secondary electrons that give us the images we see
- Teacher do you work with something else???????
Bugscope Team yes, some researchers look at compounds, or metals, or bone scaffolds, etc.
- Bugscope Team liquid nitrogen is not used for the ESEM directly
- Bugscope Team in between each of the ommatidia are little spikey hairs called setae (see-tee)
- Bugscope Team we use liquid nitrogen with this microscope just to keep the field effect transistor cooll enough so that the signal to noise will be high
- Student What function does those hairs have?
Bugscope Team the hairs, called setae, are used for sensory, chemosensory or mechanosensory. the setae connect to nerves underneath the exoskeleton
- Bugscope Team the hairs on the eyes help the fruit fly judge windspeed
- Teacher which size is the butterfly?
Bugscope Team we didn't put the whole butterfly on the aluminum stub, we only cut off a piece of the wing because the scales are interesting to look at
- Student hi, again
- Student melo te amo
- Bugscope Team as Cate said we are supposed to call the hairs setae
- Bugscope Team hola again ManDuGo
- Student which part of the insect are we?
Bugscope Team this is just a part of a monarch butterfly wing
- Teacher scott what did study?
- Bugscope Team the stuff on the bottom is silver paint
- Bugscope Team it helps ground the electrons
- Bugscope Team this as Cate says is just a portion of the wing
- Teacher the hairs in the eyes help for what???
Bugscope Team the setae in the eyes are for sensing wind movement and air speed, they help the fly to respond very quickly to things in its path
- Student what kind of buttlerfly is
Bugscope Team this is a part of the wing of a monarch butterfly
- Teacher are you happy with your work scot?
- Bugscope Team the hairs on the fruit fly eyes help it judge wind speed/direction
- Teacher scott what did you study?
Bugscope Team he studied biology and english
- Teacher you are all butterflies!!
- Bugscope Team mariposas
- Bugscope Team d'oh
- Bugscope Team juan, are you logged in as "wonder boy" or is that someone else?
- Teacher are there other machines to analize insects?
- Teacher what does this butterfly eat??
Bugscope Team it eats milkweed, which is often poisonous to many other animals
- Bugscope Team I was afraid I would become an English teacher so I added biology
- Teacher that is someone else..sorry for that comment...\
- Bugscope Team ah, no problem juan, we were all kids ourselves one day ;)
- Bugscope Team you could use a light microscope, of which there are many types, to study insects
- Bugscope Team since the butterfly likes to eat from milkweed, other predators won't eat it because it is poisonous from eating it as well
- Student so tell me, what are we exactly trying to see?
- Bugscope Team we have several cool microscopes in our lab: electron, atomic force, confocal, reflected, etc.
- Student how fast does the butterfly is able to f
- Bugscope Team here we see the scales of the Monarch butterfly, but we are at low mag...
- Student hey i have a question, do we have this kind of technology in colombia?
- Bugscope Team the colors of the scales are apparently not real colors but structural colors
- Student what is vacuum used for during the process?
- Bugscope Team it is very likely you have several of these microscopes in Colombia
- Teacher what is this?
- Teacher which is your function?
- Bugscope Team the vacuum ensures that the electrons can fly unimpeded through space
- Bugscope Team or smaller
- Bugscope Team the scalebar is in the lower left so you can see right now is is saying 1mm
- Student was it dificult to upload this sistem to internet??
- Bugscope Team electron microscopes are specialized CRTs.
- Bugscope Team we wrote the first control software over 9 years ago
- Teacher when did you start this virtual system and why?
- Teacher where does it lives?
- Student what does CRTs means?
- Bugscope Team Chas rewrote it recently
- Student what is CRTs?
Bugscope Team CRT means cathode ray tube
- Bugscope Team crt is a cathode ray tube, which is what a TV is
- Student which is the fuction of the hair in the bugs?
Bugscope Team the hairs are called setae (see-tee), and they are connected to nerves underneath the exoskeleton, they help the insect to sense its environment
- Student is this the mos powerful microscope ever made?
- Bugscope Team but in this case the cathode is controlled so that the electron beam can be collimated and directed,using scanning coils, in a pattern at the sample
- Bugscope Team there are now more powerful microscopes, but this is a particularly good one
- Bugscope Team setae (hairs) can be chemosensory or mechanosensory, so they can help the insects sense things in many cool ways
- Student how many people are you able to have conected at the same time???
Bugscope Team bugscope can take 25-30 people maximum, more than that and the lag is so bad. it also depends on your network connection, if you are fast, then we can take more
- Student how mucvh does a microscope like this cost
- Student how much is one of those microscopes?
- Bugscope Team when you have a more powerful microscope, as in better resolution, you have to also give up something, like the size of the sample or the depth of field
- Bugscope Team this cost about $600,000 in 1998
- Bugscope Team we don'e to have anymore than 20, it tends to create a big lag sometimes as well as a lot of confusion when they are all typing at once
- Bugscope Team but our dollar is not what it used to be!
- Teacher what is the capacity of this microscope?? the maximun zoom?
- Bugscope Team we can actually magnify over 800,000x
- Student are the pictures shown by the electronic microscope all in black and white?
Bugscope Team yes, it is all black and white because the image is gathered with electrons, not light, but we can false color the images afterward, using elemental analysis of the image
- Bugscope Team but for useful publishable images no more than about 200,000x
- Student so, is that the bugs mouth?
- Bugscope Team the images are black and white because we are not using light to collect the images -- we are using electrons
- Bugscope Team in the middle of the image looks to be a palp, which helps the insect taste or eat food
- Bugscope Team electrons are smaller than the wavelengths of visble light (400 to 700 nm)
- Student byer vhsdf\
- Student bye\
- Student hi!\
- Student well thanks for the help
- Student bye thank for your help\
- Student good luck
- Bugscope Team gracias, you all rocked and or rolled!
- Student you! sorry!
- Student see you
- Student later
- Bugscope Team Thank You, It was a pleasure for us as well
- Student thank you very much for your information...
- Student thank you! it was a great experience
- Bugscope Team hasta luego, amigas y amigos!!
- Student what type of lenses does the microscope use????
- Bugscope Team come back again!
- Student hasta luego que esten bien!
- Bugscope Team electromagnetic lenses
- Bugscope Team juan, all the images and chat are saved on your bugscope member page, your students can look at it anytime: http://bugscope.beckman.uiuc.edu/members/2008-023
- Teacher the guys are now leaving..sorry for the inconvenients...it was really an exciting experience
- Bugscope Team coils of wire ; )
- Student bye thnks
- Bugscope Team no inconvenience for us -- this is fun for us
- Bugscope Team many thanks to our amigos y amigas in cali colombia
- Bugscope Team Thanks ManDuGo.
- Teacher thanks again for making this possible...I'm leaving now...
- Teacher I'll send you some postcards from cali colombia..;)\
- Bugscope Team Gracias Juan!
- Bugscope Team Awesome.
- Bugscope Team we would love to see some pics!
- Bugscope Team adios juan
- Bugscope Team write us if you need anything
- Bugscope Team okay, looks like juan is gone, we'll start closing the session now.
- Bugscope Team Thanks Alexsan
- Bugscope Team And Angel C
- Bugscope Team okay, session locked, disabled, rxl stopped
- Bugscope Team what about Guest?
- Bugscope Team litle nerdie, guest, we have to say good bye now
- Bugscope Team and little nerdie?
- Bugscope Team well, did guest want to drive?
- Bugscope Team user is here
- Bugscope Team nice session everyone, good bye!